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Microscope System

Jan 2013
Carl Zeiss Microscopy LLCRequest Info
For 3-D fluorescence imaging, Carl Zeiss Microscopy LLC has introduced a new microscopy technology. Lightsheet Z.1 provides biologists with a new method of imaging dynamic processes in living organisms.

Biologists can use it to observe the development of organisms over several days or more. The low phototoxicity and the integrated incubation provide insight into the differentiation of cell groups without harming the specimen. On large objects, such as fruit fly or zebra fish embryos, the light sheet microscope delivers more information than established methods of fluorescence microscopy. The system can also be used in marine and cell biology, and plant physiology.

It works with an expanded light beam, the light sheet, which illuminates only a thin section of the sample, thus protecting the rest of the specimen. Images are captured at a 90° angle to the light sheet. The system achieves maximum image quality at minimal illumination intensity and is suited for long-term examinations of living specimens. Multiview imaging enables data acquisition from different viewing angles. These can be combined through mathematical algorithms into 3-D reconstructions and time-lapse videos.

The light sheet system of the microscope uses a new type of optical concept that combines cylindrical lens optics with laser scanning. Users receive homogeneously illuminated optical sections of complete examination objects.


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3-D fluorescence imaging3-D reconstructionAmericasBiophotonicsBreakthroughProductsCarl Zeisscell biologycylindrical lens opticsexamine living specimensfruit fly embryosimage dynamic processeslaser scanninglenseslight sheet microscopeLightsheet Z.1marine biologyMicroscopymultiview imagingNew Yorkobserve development of organismsopticsplant physiologyProductstime-lapse videozebra fish embryos

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