Search Menu
Photonics Media Photonics Marketplace Photonics Spectra BioPhotonics Vision Spectra Photonics Showcase Photonics ProdSpec Photonics Handbook
Promoted content

Multi-Immersion Objectives

Applied Scientific Instrumentation Inc.Request Info
Facebook Twitter LinkedIn Email
Multi-Immersion ObjectivesASI and Special Optics developed two objective lenses for light sheet imaging of cleared tissue samples as well as live cell imaging. Because of their unique curved first surface, both objectives work with all media regardless of refractive index — air, water, glycerol, oil, and cleared tissue media all work equally well. A common problem in live cell imaging is spherical aberrations as imaging goes deeper into the sample. This can be reduced by matching the medium refractive index to the sample refractive index.

However, there are residual aberrations from using e.g. a water dipping objective with non-water medium. This aberration is eliminated by using these objectives. The 54-10-12 objective has an NA of 0.4, 17x magnification, 12 mm working distance, and 1.2 mm field of view. The 54-12-8 has an NA of 0.7, 24x magnification, 10 mm working distance, and 1.0 mm field of view. The actual NA and magnification are both proportional to the immersion medium refractive index; stated values are for RI 1.45.

The long working distance and relatively slender profile of these objectives is critical for light sheet imaging, and stands in contrast to other cleared tissue objectives which, despite their long working distances, cannot be co-focused because of their wide barrels. When used in multi-view light sheet like the ASI diSPIM, the objectives offer 800 nm and 450 nm resolution respectively in X, Y, and Z in FocusClear (CLARITY) solution. The fluidic sealing has been engineered to allow the objective lenses to be dipped into harsh chemicals including DBE, BABB, ECi, CUBIC, and FocusClear.

     —   Dual-view cleared tissue imaging, e.g. ct-dSPIM
     —  Single-view cleared tissue imaging, e.g. COLM/OpenSPIM/ct-ASLM setups
     —   Live cell imaging with RI-matched media
     —   Any imaging requiring very long working distances with modest NA

* First Name:
* Last Name:
* Email Address:
* Company:
* Country:

When you click "Send Request", we will record and send your personal contact information to Applied Scientific Instrumentation Inc. by email so they may respond directly. You also agree that Photonics Media may contact you with information related to this inquiry, and that you have read and accept our Privacy Policy and Terms and Conditions of Use.

Register or login to auto-populate this form:
Login Register
* Required

Microscopylight sheet microscopyCleared Tissue SamplesDual Selective Plane Illumination MicroscopySub-Micron ResolutionSPIMdSPIMMicroscope Cleared Tissue Objective

back to top
Facebook Twitter Instagram LinkedIn YouTube RSS
©2023 Photonics Media, 100 West St., Pittsfield, MA, 01201 USA, [email protected]

Photonics Media, Laurin Publishing
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.