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Multi-Immersion Objectives

Applied Scientific Instrumentation Inc.Request Info
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Multi-Immersion ObjectivesASI and Special Optics have developed two dipping objective lenses designed for light sheet microscopy of cleared tissue samples, including ASI’s ct-dSPIM. These objectives work in any refractive index media without a correction collar because of a unique curved first surface. They are robust to immersion in harsh media including DBE and BABB. The original objective lens (54-10-12) has nominal NA of 0.4, WD of 12 mm, and allows imaging over 5 mm deep into a flat sample. The second one (54-12-8) has an increased NA of 0.7 with a WD of 10 mm. Both NA and magnification vary slightly with the refractive index of the immersion medium, but WD is constant.

Like other ASI components, these objective lenses are available for sale individually or as part of complete systems. More objective lenses for light sheet microscopy are planned; inquire for details. Applications:
  • Dual-view cleared tissue imaging (diSPIM with cleared tissues)
  • Single-view cleared tissue imaging (OpenSPIM setups for cleared tissues)
  • Water-dipping applications with very long working distances

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    ProductsMicroscopylight sheet microscopyCleared Tissue SamplesDual Selective Plane Illumination Microscopycleared tissue objectiveSub-Micron ResolutionSPIMdSPIMmulti-immersion objectivesPBG3

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