Close

Search

Search Menu
Photonics Media Photonics Buyers' Guide Photonics EDU Photonics Spectra BioPhotonics EuroPhotonics Industrial Photonics Photonics Showcase Photonics ProdSpec Photonics Handbook
More News

Neurobiotin 488 Tracer Molecule


 
Facebook Twitter LinkedIn Email Comments
BURLINGAME, Calif., Aug. 31, 2012 — The Neurobiotin 488 tracer trifunctional molecule from Vector Laboratories Inc. is designed for neuronal tracing and cell filling.

A bright-green fluorophore, it is similar in fluorescence to fluorescein, Cy2, Alexa Fluor 488 and DyLight 488. It features a biotin label with a biotinidase-resistant linkage and a fixable primary amine.

It is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. It is readily fixed with standard aldehyde-based fixatives. The tracer can be viewed directly with fluorescence microscopy and can be detected and amplified via the biotin moiety with fluorescence or enzyme-based avidin or streptavidin detection systems.

In a neuron of the dorsal motor nucleus of the vagus of the brainstem in a mouse brain slice, Neurobiotin 488 was used for cell filling to identify the neuron from which patch clamp recordings were taken.

For more information, visit: www.vectorlabs.com.


Photonics.com
Aug 2012
MORE FROM PHOTONICS MEDIA
Biophotonics
aldehyde-based fixativesAlexa Fluor 488AmericasavidinBiophotonicsbiotin labelbiotin moietybiotinidase-resistant linkagebright green fluorophoreCaliforniacell fillingCy2DyLight 488fixable primary aminefluoresceinfluorescence microscopyMicroscopyNeurobiotin 488neuronal tracingpatch clamppressure injectionProductsstreptavidintracer moleculetrifunctional moleculeVector Laboratories

back to top
Facebook Twitter Instagram LinkedIn YouTube RSS
©2019 Photonics Media, 100 West St., Pittsfield, MA, 01201 USA, info@photonics.com

Photonics Media, Laurin Publishing
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.