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Red Fluorescent Intercalating Dye

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Red Fluorescent Intercalating DyeThe EvaRuby red fluorescent intercalating dye from Biotium is designed for qPCR and HRM® techniques in life science research.

The dye can be incorporated into probe-based qPCR assays, expanding multiplexing options for probe-based qPCR. EvaRuby dye is compatible with ~470-nm excitation and ~610-nm emission filters. It features a Stokes shift of 480/613 nm, allowing excitation of EvaRuby dye by the same green/blue channel as EvaGreen® dye and detection in the orange channel. As a result, EvaRuby dye has minimal cross-talk in standard probe detection channels and enables the combination of dye-based and probe-based qPCR in a single reaction.

Jan/Feb 2022

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