Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a very versatile microscopy method where fluorescence lifetime information is combined with spatial localization in the sample, allowing investigating, for example, biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition is typically based on Time-Correlated Single Photon Counting (TCSPC) electronics, pulsed diode lasers, and highly sensitive single photon counting detectors. Up to now TCSPC...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. The integration can overlay both FLIM and Raman images...Promoted Content December 2022 IssueNEW 4-Wavelength LED IlluminationCoolLED has launched its new pE-400 Series Illumination Systems where sophisticated control combines with powerful illumination for cost-effective fluorescence microscopy, from routine to advanced...BioPhotonics Sep/Oct 2022 IssueLaser Imaging MicroscopeThe HYPERION II FT-IR Laser Imaging Microscope from Bruker Corp. combines FT-IR microscopy with quantum cascade laser IR imaging for R&D purposes. The system provides all three...Promoted Content January 2023 IssueSingle-Objective Light SheetThe single-objective light sheet geometry – known in the literature as OPM, SCAPE, SOPi, eSPIM, and SOLS – enables 4D fluorescence imaging of biological samples at high speed and low...ARTICLESBioPhotonics Nov/Dec 2022 IssueFlow Cytometry Technique Shows Promise for Early Cancer DiagnosisA digital droplet microfluidic flow cytometry technique could enable earlier recognition of tumor development and progress, by aiding in the detection of multiple microRNAs (miRNAs) in single...BioPhotonics May/Jun 2023 IssueStress tests borne of mice and menMen and women respond differently to stimuli, and their hearts respond differently, too. Scientists are continuing to develop an understanding as to why these disparities in physical reactions exist....BioPhotonics Nov/Dec 2022 IssueExpansion Microscopy Technique Reveals Hidden NanostructuresProteins and other molecules are often tightly packed together inside a living cell. These dense clusters can be difficult to image because the fluorescent labels used to make them visible...BioPhotonics Jan/Feb 2024 IssueLabel-Free Imaging Shows Dynamics of Intracellular Cargo TransportUntil now, scientists have relied on fluorescence microscopy techniques to study intracellular cargo transport, a vital process to maintaining essential cellular functions. However, the effects of...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookSuperresolution Microscopy: An Imaging RevolutionSuperresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookNanopositioning: A Step AheadBy its original definition, a nanopositioning device is a mechanism capable of repeatedly delivering motion in increments as small as one nanometer. Lately demands from industry and research have...Photonics HandbookImage Intensification: The Technology of Night VisionImage intensification, the basis of night vision, is a complex conversion of energy particles that occurs within a vacuum tube. An image-intensifier system works by collecting photons through an...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 2/28/2024Multi-Bandpass Filters for FluorescenceLearn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.Photonics.com 2/21/2024Cooling SWIR Sensors: an OverviewDark current is a critical parameter when one is looking to acquire a scientific imaging camera, especially in the short-wave infrared (SWIR) region. Very careful attention must be paid to the...Photonics.com 10/11/2022Cricket 2 Single MCPAdvanced Image Intensifier adapter for low light level imaging applications, the Cricket™² is a plug & play camera attachment enabling low light level imaging and extreme high shutter speeds for CMOS...WEBINARSPhotonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. Emphasis is placed on the integration of FLIM in surgical...Photonics.com 10/26/2022Getting Outstanding Results From Multispectral TCSPC Fluorescence Lifetime ImagingHeitz discusses how scientific results from various cutting-edge studies performed by Becker & Hickl and the company’s collaborators were generated and what to consider when planning...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarysingle-photon avalanche diodeA single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical signals, down to the level of single photons. SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionaryelectron multiplication charge-coupled device cameraAn EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for low-light imaging applications that require high sensitivity and fast readout...Photonics Dictionarytwo-photon excited fluorescenceTwo-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living tissue. The advantage of TPEF in comparison to conventional fluorescence...VIDEOSPhotonics.com 10/12/2021pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 1/18/2018Supporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 11/20/2023Andor's Life Science Research SolutionsAt Andor, it matters to us that the products we develop make a real difference to our customers and contribute significantly to their research across Immunology, Microbiology, Neuroscience, Cells &...PHOTONICS BUYERS' GUIDE CATEGORIESPicosecond LasersAutocorrelatorsAn autocorrelator is a signal-averaging device that improves signal-to-noise ratio by comparing a sampled signal with a time-delayed form of itself.Streak CamerasA camera streak is a high-speed cine camera used to record, on a continuously moving film, very brief events such as a flash of light. The image is recorded as a continuous image, not as a series of...Scintillation DetectorsCOMPANIESPhotonics Buyers' GuidePhotron USA Inc.Photron has been a global market leader in high-end, high-speed camera systems for over forty years. The company’s continuing development of new state-of-the-art products illustrates a...Photonics Buyers' GuideLaserPoint srlLaserPoint manufactures a wide portfolio of laser power and energy meters able to perform laser measurements with high accuracy. They developed the hard coatings to absorb the laser radiation which...Photonics Buyers' GuideDK Photonics Technology LimitedDK Photonics is a leading manufacturer of innovative and high quality optical passive components. We provide complete and integrated solutions from 400~2100nm. DK Photonics produces a large array of...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...