Request InfoApplied Scientific Instrumentation Inc.The objective has been designed for light sheet microscopy of cleared tissue samples. It’s used with any ASI SPIM configuration, enabling isotropic resolution without manipulating the sample. The objective offers submicron resolution in X, Y, and Z when used with a multiple-view system. It permits imaging >5 mm deep into flat samples, or up to 12 mm into spherical samples.See full productRelated content from Photonics MediaPRODUCTSPhotonics Showcase February 2021 IssueMicroMirror TIRF MicroscopeThe Mad City Labs RM21® MicroMirror TIRF microscope is a unique singlemolecule microscope. Spatial separation of the excitation and emission beams and the use of micromirrors leads to superior...Photonics Showcase March 2022 IssueFlat Top Narrowband FiltersAlluxa’s Ultra Series Flat Top Narrowband Filters offer the narrowest bandwidths and squarest filter profiles in the visible and NIR, with FWHMs of 0.25 nm to 2 nm, transmission levels >90%...Photonics Showcase July 2021 IssueFemtoFiber ProThe FemtoFiber Pro product family consists of versatile and high-performance femtosecond fiber lasers. They feature a mode-locking mechanism based on saturable absorber mirrors and use polarization...Photonics Showcase February 2021 IssueMulti-kW Laser Beam ProfilingCCD chips can now survive exposure to multi-kW laser powers with the Ophir® LBS-300HP-NIR Beam Splitter. An industry first, this affordable, compact device reduces extremely high laser power an...ARTICLESBioPhotonics May/Jun 2022 IssueThree-Photon Microscopy Reveals Immune Cell Behavior in Real TimeA research group from Cornell University has used three-photon microscopy to image the full depth of the popliteal lymph node in a living mouse — reportedly for the first time. The researchers...Photonics Spectra March 2022 IssuePhoton-Controlled Electron Beams Push Bounds of Quantum MetrologyThe combination of integrated photonics and electron microscopy supports a method for highly efficient electron-beam modulation. The experimental work described by scientists at École...Photonics.com 2/5/2021Donovan Leonard Named Physical Science Director for Microscopy Society of AmericaDonovan Leonard, a researcher in the Deposition Science and Technology group at Oak Ridge National Laboratory (ORNL), has been elected to a three-year term as the physical sciences director for the...Photonics.com 7/5/2022High-Throughput Imaging Links Microbial Metabolism to IdentityAn imaging platform for investigating microbiomes in medical and environmental samples can perform high-throughput metabolism and identity analyses with single-cell resolution. Called SRS-FISH, or...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookMicroscopy Illumination: Considering Nonlaser Light Sources: Metal halide and LED light sources are changing the illumination landscape in microscopy. Brighter, easier to use and growing in application, they are driving new options in fluorescence, conventional bright-field and other contrast techniques.As recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...Photonics HandbookInterferometry: Measuring with Light: Long applied to optical shop testing, interferometry is now used to measure many different types of parts in a variety of applications, such as optical system performance, surface roughness, surface form, and displacement of moving surfaces.An interferometer is an instrument that compares the position or surface structure of two objects. The basic two-beam division of amplitude interferometer components consists of a light source, a...Photonics HandbookMeasuring Surface Roughness: The Benefits of Laser Confocal Microscopy: 3D imaging and ultrahigh resolution are ideal for the inspection of materials with defined surface finishes and textures.When evaluating the surface of a component, surface roughness can be assessed by eye or by rubbing it with a fingertip. Common expressions include “shiny,” “lusterless and...Photonics HandbookNSOM: Discovering New Worlds: Near-field scanning optical microscopy (NSOM) is an optical microscopy technique capable of imaging objects with a resolution below the diffraction limit of conventional (far-field) microscopy; i.e., below approximately half the wavelength of the light used.NSOM is suitable for studies on the mesoscopic scale (several tens to hundreds of molecular dimensions). It has become an important tool in research and applications of semiconductors, organic layers...WHITEPAPERSPhotonics.com 2/28/2022Spatial Light Modulator 1024 x 1024The 1024 x 1024 High-Speed Spatial Light Modulator is a beacon of advanced SLM technology and proprietary Meadowlark Optics software. The datasheet goes in-depth on its remarkable phase stability,...Photonics.com 11/17/2020High-Speed Imaging in Biomedical Microfluidic ResearchThis whitepaper discusses multiple case studies when Phantom cameras have assisted in the advancement of microfluidic and cellular research.Photonics.com 12/10/2021Cutting Edge Measurement of an InsertThe 3D optical profilometer S neox Five Axis makes it possible to quickly and flexibly obtain the key parameters necessary for the continuous improvement of our tools, such as the cutting edge...Photonics.com 3/5/2021Atomic Force Microscopy Enhances the Nanoscopy ToolkitImaging and morphology represent a critical puzzle piece when trying to understand nanoscale structures, whether they are naturally occurring, such as viruses, or engineered structures, such as...WEBINARSPhotonics.com 1/13/2022New Industrial Challenges for Optical Metrology as Autonomous Cars, Free-Space Optical Communication, and AR/VR Shape the FutureA portion of the optical manufacturing market is driven by consumer electronics such as smartphones, augmented/virtual reality (AR/VR) devices and automated driving via advanced driver-assistance...Photonics.com 10/26/2021Quantitative Analysis of Intact Organs Using Adaptive Light-Sheet MicroscopyAlon Greenbaum discusses methods for quantitative analysis of intact organs using tissue clearing and adaptive light-sheet microscopy. As a test case, he presents macroscopic and microscopic...Photonics.com 10/18/2018Continuously Variable Filters for Spectroscopy, HSI, and Fluorescence DiagnosticsThis webinar will introduce you to advances in variable filters, especially in the areas of transmission and blocking. You will learn about the properties and performance of variable filters, and how...Photonics.com 10/29/2014Imaging Biology at High Spatiotemporal ResolutionJoin us for a FREE webinar Wednesday, Oct. 29, 2014, 1 p.m. EST Presenter Dr. Hari Shroff will discuss efforts to develop high-resolution optical methods better suited than conventional imaging...PHOTONICS DICTIONARY+ TERMSEDU.photonics.com 3/5/2018Nomarski microscopyAlso referred to as differential interference contrast (DIC) microscopy; Nomarski microscopy is a unique form of microscopy especially suited for the evaluation of surface quality and defects as well...EDU.photonics.com 6/25/2019quantum cascade laserA Quantum Cascade Laser (QCL) is a type of semiconductor laser that emits light in the mid- to far-infrared portion of the electromagnetic spectrum. Quantum cascade lasers offer many benefits: They...EDU.photonics.com 6/30/2017tip-enhanced Raman spectroscopyA spectroscopic technique that combines the high chemical specificity of Raman scattering and signal sensitivity provided by surface plasmon resonance enhancement, with the nanoscale spatial...EDU.photonics.com 9/8/2009beta fluorographyThe use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials that emit visible fluorescence during electron bombardment. The method is...VIDEOSPhotonics.com 1/24/2022Welcome to CoolLEDIntroducing the experts who design and manufacture high performance LED Illumination Systems.Photonics.com 5/2/2022Applied Scientific InstrumentationEstablished in 1990, Applied Scientific Instrumentation is a reknown American company committed to the advancement of science. ASI products have been thoroughly tested and evaluated in numerous labs...Photonics.com 10/12/2021pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 5/5/2017Lasers & Bacteria: Saving Lives - LIGHT MATTERS 04.2017Millions of unexploded landmines and ordnance from World War II are still buried in countries around the world, injuring hundreds of thousands of people every year. Now, efforts to detect and remove...PHOTONICS BUYERS' GUIDE CATEGORIESAutomated MicroscopesPetrographic MicroscopesA petrographic microscope is a microscope equipped with a polarizer, an analyzer and a Bertrand lens to focus on the upper focal plane of the objective. It is used chiefly by mineralogists and...Bioluminescence MicroscopesMicroscopy NanotechnologyCOMPANIESPhotonics Buyers' GuideApplied Scientific Instrumentation Inc.Applied Scientific Instrumentation, Inc. (ASI) is a company committed to the advancement of science. Our engineers and technicians have years of experience in aiding researchers with their technical...Photonics Buyers' GuideAndor TechnologyWe are global leaders in the development and manufacture of high performance scientific digital cameras for academic, industrial and government applications. Through continuous dialogue with our...Photonics Buyers' GuideOxxius SAOxxius develops and manufactures DPSS (Diode Pumped Solid State) lasers and laser diode modules in the ultraviolet, visible, and near-infrared wavelength ranges. The LCX series of DPSS lasers and the...Photonics Buyers' GuideMad City Labs Inc.Mad City Labs, Inc. is a leading manufacturer of piezo nanopositioning systems and instrumentation capable of sub-nanometer positioning resolution. Our product line of nanopositioners,...