Time-resolved Fluorescence Microscope with Super-resolution Capability

PicoQuant GmbH

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Picoquant Time-resolved Fluorescence microscope

The MicroTime 200 STED is a complete confocal STED system with unlimited flexibility for time-resolved applications.

Features:
A popular technique for super-resolution imaging is Stimulated Emission Depletion (STED) microscopy and is ideally suited for integration into confocal microscopes. Highest robustness and ease-of-use were the main focus when integrating this method into the MicroTime 200. Thus STED microscopy with spatial resolutions down to 50 nm can be performed without lengthy alignments while the system retains the full flexibility and capabilities of our open microscopy platform design. A dedicated laser combining unit provides a choice of excitation lasers with wavelengths at 595, 640, and 660 nm along with the depletion laser at 766 nm. The detection subsystem can be configured with up to four individual single photon sensitive detectors. The powerful, yet easy to use SymPhoTime64 software features dedicated STED data acquisition and analysis methods, including a unique fluorescence pattern matching approach for the separation of multiple species from a single wavelength excitation STED measurement.

Application:
The MicroTime 200 STED supports many time-resolved measurement and analysis procedures such as fluorescence lifetime imaging (FLIM), fluorescence correlation spectroscopy (FCS), fluorescence lifetime correlation spectroscopy (FLCS), and Förster resonance energy transfer (FRET) to name just a few. All supported methods can be carried out at either confocal or STED resolution and even simultaneously by using pulsed interleaved excitation (PIE).

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