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VICTOR, N.Y., July 6, 2010 — Till Photonics Inc.’s iMIC-Andromeda imaging workstation combines a broad range of imaging applications. The modular approach enables integration of methods such as total internal reflection fluorescence, fluorescence recovery after photobleaching, photoactivation, epifluorescence, Förster resonance energy transfer, multiphoton, structured illumination and laser spinning disk confocal microscopy.

The heart of the Andromeda is a single spinning disk that is based on a new optical concept. Instead of using microlenses and two synchronized rotating disks, it requires only a single disk and places long-pass dichroics into the infinity space. This greatly improves the point spread function and provides higher resolution and better optical sectioning than achieved by previous approaches.

The core component of Andromeda is a patented optical design that comprises a quasihexagonal pattern of concave micromirrors carrying pinholes in their center. While the micromirrors form a rotating point pattern, a retroreflector projects the focus pattern back onto the pinholes where it can pass and reach the sample.

This approach ensures maximal optical throughput and enables a much better matching to the numerical aperture of a variety of objectives, including water-immersion and low-magnification types.

Features include a modular concept for more configurations, automation to control complex experiments, C-mount, flexible software for changing needs, improved smoothness of rotation and integration of all major fluorescence techniques.

The system scans at up to 10,000 rpm and is field-optimized for 9 × 9-mm CCD chips. It provides full motorized switching of all filters (ten excitation filters, ten emission filters and five dichroic mirrors). At 60×, axial resolution is <800 nm, and lateral resolution is diffraction-limited. Excitation wavelength range is from 400 to 700 nm.

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Jul 2010
AmericasBiophotonicsC-mountCCDconcave micromirrorsepifluorescenceFiltersfluorescence recovery after photobleachingFörster resonance energy transferFRAPFRETimagingimaging workstationiMIC-Andromedainfinity spacelaser spinning disk confocallong-pass dichroicsMicroscopymirrorsmodularmultiphotonopticsphotoactivationpinholesProductsquasi-hexagonalretroreflectorsingle diskstructured illuminationTILL PhotonicsTIRFtotal internal reflection fluorescence

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