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Confocal Raman Microscopy: A Clinical and Biological Tool
Scientists in the fields of biology and biochemistry are familiar with some of the more common forms of spectroscopic analysis, such as absorption and emission spectroscopy. The former measures the amount of light absorbed at a given wavelength to...
BioPhotonics, October 2006
In Vivo Experimental Imaging of Complex Disease Processes
In vivo studies are the only way to accurately assess the interactions of multiple cell types in the context of complex anatomical features. Cancer and inflammatory disease, for example, represent the combined processes of cell migration and...
BioPhotonics, October 2006
Lasers Stimulate New Techniques in Nerve Studies
Neural tissue stimulation is used in research and clinical applications. Neuroscientists use nerve stimulation to answer fundamental questions about the function of the nervous system and to research diseases such as Parkinson’s and...
BioPhotonics, October 2006
FRET Ventures Out of the Lab
Förster resonance energy transfer (FRET) offers a unique means of visualizing biological activity. The technique uses two molecules, a donor and an acceptor. Upon excitation, if the molecules are close to one another, the donor transfers its...
BioPhotonics, September 2006
Nanobarcodes Check Out Genes
Genes carry information that controls the physical development and behavior of every biological organism. Gene expression changes dynamically in living cells and manifests as patterns with numerous combinations, reflecting the physiological states....
BioPhotonics, September 2006
Optical Traps Illuminate the Subcellular World
Optical trapping has been utilized in endeavors as varied as microfabrication, particle sorting and the study of nonequilibrium thermodynamics. The technique, also known as optical tweezing, is particularly beneficial for studies on the cellular...
BioPhotonics, September 2006
Watching Golgi Maturation
Within cells, the Golgi apparatus gets things where they need to go. An organelle found in all eukaryotic organisms, the Golgi apparatus primarily takes in protein-containing vesicles, then modifies, sorts and ships them off to their final...
BioPhotonics, September 2006
A Medical Diagnostic Application of Raman Multimodal Multiplex Spectroscopy
The new and rapidly expanding field of nanoscale medical diagnostics requires the development of advanced sensor technologies to provide the selectivity and sensitivity needed to measure concentrations of target biomarker molecules at subnanomolar...
BioPhotonics, August 2006
Getting to the Core
At Harvard Medical School in Boston, Jennifer Waters is quite popular at times, especially when she’s running a one-day training workshop for postdoctoral researchers. She and her staff teach the course every two weeks, and that isn’t...
BioPhotonics, August 2006
Improving Capillary Electrophoresis the Photonic Way
As a tool for divining all that is knowable about proteins, capillary electrophoresis has taken a strong grip on investigators. The technique offers scientists from oncologists to pharmacologists speedy and sensitive separation of proteins —...
BioPhotonics, August 2006
Medical Imaging Learns from Computer Gaming
Medical imaging systems such as CT, MRI and ultrasound are becoming ubiquitous. As the technology becomes more commonplace, it also continues to mature. Significant imaging applications arise each year to aid in the diagnosis and monitoring of...
BioPhotonics, July 2006
Resolution of Optical Microscope Redefined
Optical microscopy is an important tool for studying live biological cells in life sciences research, enabling the observation of various biological processes in real time. The study of nanoscale single molecular activities such as protein-protein...
BioPhotonics, July 2006
Spectral Confocal Microscopy Aids FRET Studies
FRET is an energy transfer between two fluorescent molecules that occurs without the exchange of a photon. It occurs when a fluorescent molecule, usually called the donor probe, is excited by the absorption of a photon. If a fluorescent probe that...
BioPhotonics, July 2006
Direct Proximity Imaging with FRET and FLIM
Florescent proteins have opened the possibility to observe protein distribution and localization by fluorescence microscopy. Directly observing these nanometer-size molecules is not possible, however. As an alternative, indirect methods such as...
BioPhotonics, June 2006
Eye Tracking Helps Improve Accuracy in Radiology
Improving the accuracy of radiology diagnostic reading is critical for a number of reasons. Greater accuracy means that a higher percentage of malignancies will be detected — and detected earlier — increasing the chances for a complete...
BioPhotonics, June 2006
Microscope Takes Deep-UV and Visible Picosecond Fluorescence Lifetime Measurements
Time-resolved microscopy is the ultimate tool for investigating dynamic events in cells and subcellular structures. However, it previously was limited when used for applications that required wavelengths longer than 370 nm because of microscope...
BioPhotonics, June 2006
Multispectral FLIM Enhances FRET Autofluorescence Imaging
er the past decade, confocal and multiphoton laser scanning microscopy have become standard for biomedical imaging on the cellular level. Because these techniques either suppress or don’t generate out-of-focus light, they produce clear images...
BioPhotonics, June 2006
On the Path Toward More Useful Fluorophores
Where would modern biomedical research be today without fluorescent probes? Some would argue that it would be blindly groping down a dark alley, with no efficient means of discovering the finely honed interplay of proteins and other substances in...
BioPhotonics, June 2006
Camera Phones Emerge as a Health Care Tool
Telemedicine gives patients access to a doctor no matter where they are. Whether a patient uses monitoring equipment to send information to the doctor’s office or an x-ray technician transmits images to a radiologist for examination,...
BioPhotonics, May 2006
Creating Clarity: Adaptive Optics for Bioimaging
Biological imaging instruments often have resolution limitations that restrict the ability of researchers and clinicians to detect critical detail. One reason is that, as light passes through tissue to reach the object of interest — a cell,...
BioPhotonics, May 2006
Focusing on the Experiment
For Alexey Khodjakov, a researcher at the New York State Department of Health’s Wadsworth Center in Albany, it wasn’t a question of if but when he would lose focus. Following cells as each divided into many, he and colleagues in his lab...
BioPhotonics, May 2006
A Spotlight on Biophotonics in Canada
Canada has demonstrated its commitment to building a sustainable future for biophotonics through education, research and industrial development. Numerous initiatives exist, at federal and provincial levels, whose mandate is to establish a firm...
BioPhotonics, April 2006
Protein Interactions Profiled Using Surface Plasmon Resonance
Proteins are the worker bees of the human body. They traverse cell membranes to receive and propagate signals, transport oxygen to every cell in the body, regulate the expression of genes and impart structure and strength to tissues. The signaling...
BioPhotonics, April 2006
Virtual Microscopy Slides into Place
Palos Pathology Associates Ltd. provides pathology services to a 300-bed community hospital in suburban Chicago, and Dr. Stephen G. Ruby, president of the company, is thinking of expanding the geographic area that it covers. In the past, that would...
BioPhotonics, April 2006
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