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fluorophore Dictionary Terms

Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
nanoparticle photonic resonator absorption microscopy
Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...
single molecule localization microscopy
Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
superresolution
Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...
fluorescence lifetime
Fluorescence lifetime refers to the average time it takes for a fluorophore, a molecule that absorbs light at one wavelength...
single-photon avalanche diode
A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
time-correlated single photon counting
Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...
stochastic optical reconstruction microscopy
Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of...
autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...
single-molecule FRET
Single-molecule Förster resonance energy transfer (smFRET) is a specialized technique in biophysics and molecular...
photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...

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