Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration...

Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...

Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...

Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...

Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...

Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...

Single-molecule Förster resonance energy transfer (smFRET) is a specialized technique in biophysics and molecular...

Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...

A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...

STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...

Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...

Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...

Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...

Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...

A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...

Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...

Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of...