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Biophotonics White Papers
Effects of Sterilization Methods on Key Properties of Specialty Optical Fibers Used in Medical Devices
Optical fibers with different types of polymer coatings were exposed to three sterilization conditions: multiple autoclaving, treatment with ethylene oxide and treatment with gamma rays. Effects of different sterilization techniques on key optical and mechanical properties of the fibers are reported. The primary attention is given to behavior of the coatings in harsh sterilization environments. The following four coating/buffer types were investigated: • (i) dual acrylate • (ii)...
Raman Spectroscopy in Crystallization
Two trends in the regulatory landscape have created new scientific opportunities and financial motivations for using process analytical technologies (PAT) in small molecule and biopharmaceutical manufacturing. One critical quality attribute of a...
Diode Lasers Enable Diverse Therapeutic Applications
Diode lasers have become the dominant laser type used in therapeutic applications. This is because these workhorse devices provide lower cost of ownership, a wider choice of output wavelength and power, compact, rugged packaging, and superior...
Modern CMOS Sensors and Their Use in Fluorescence-Based Applications
Dr. Felix Asche, Product Manager at Basler, outlines possibilities and application areas of modern CMOS sensor technology in medicine and science. System manufacturers whose devices are equipped with CCD sensors and who are therefore increasingly...
rapidFLIM: The New and Innovative Method for Ultra fast FLIM Imaging
rapidFLIM exploits recent advances in Time-Correlated Single Photon Counting (TCSPC) electronics, where ultra-short dead times allows imaging fast, dynamic processes via Fluorescence Lifetime Imaging (FLIM). With this new approach, FLIM image...
OptSim Circuit: A SPICE-Like Photonic Simulator for Scalable Photonic
The time for large-scale photonic integrated circuits (PICs) has arrived. Governments, academia and industry are all onboard to transfer knowledge gained from decades of manufacturing experience in electronic integrated circuits to photonic...
How Machine Vision Cameras Help Medicine
Cameras have become a significant component in a multitude of medical applications, especially in the fields of microscopy, ophthalmology, pathology, endoscopy, lab automation and biomedical research. This Basler White Paper will explain what makes...
Working with your benchtop spectrometer at high and low sample cuvette temperatures
Spectroscopy labs are home to spectrometers of varying types: UV-Vis-NIR absorbance, fluorescence, and circular dichroism, for example, all sitting on benches at room temperature. When a scientist wants to measure spectral properties of a sample at...
Thin-Film Optical Components for Use in Non-Linear Optical Systems
Some of the greatest recent advances seen in bio-imaging and detection are due to techniques that utilize non-linear optical (NLO) phenomena. These techniques have led to a Nobel prize, super-resolution images, label-free visualization of naturally...
Next Generation Thin-Film Optical Filters for Life
Fluorescence based systems have revolutionized the way organisms, cells, and biomolecules are visualized and detected. However, challenges that are common in these instruments, such as bleedthrough, background autofluorescence, and poor...
Narrow-Line Fiber-Coupled Modules for DPAL Pumping
A new series of fiber coupled diode laser modules optimized for DPAL pumping is presented, featuring greater than 400W from a 600µm core fiber of 0.22NA. Addressing one of the key challenges in DPAL pumping, the modules achieve a spectral width of...
Ultra-Narrowband Optical Bandpass Filters with Large Format and Improved Temperature Stability
Hard coated ultra-narrowband optical filters made using modern plasma processes offer much improved transmission, temperature stability and out of band blocking as compared to legacy soft coatings. These filters are used in optical systems as...
Use of White Light Fluorescence LED in long-term Live Cell Imaging Applications
Lamps have long been used in microscopy facilities to conduct time-lapse imaging experiments. Light uniformity, stability and sample exposure are key in generating reliable data. In this study, we evaluate an LED system (X-Cite 120LED) for our...
Narrow Line Diode Stacks for DPAL
Diode pumped alkali metal vapor lasers (DPALs) offer the promise of scalability to very high average power levels while maintaining excellent beam quality, making them an attractive candidate for future defense applications. A variety of gain media...
High-power Visible Spectrum Diode Lasers for Display and Medical Applications - Beam Sources with Tailored Beam Quality and Spectral Characteristics
Improved beam shaping concepts allow coupling of red and blue diode lasers into smaller fibers. For medical applications beam sources with narrow wavelength distribution in the blue spectral region were developed. Modules up to 100W in a 400µm...
Dynamic – Dynamic Range
The dynamic or dynamic range of a CCD-camera system is a widely used term to characterize the ability of a camera system to measure or image light/dark differences. In the field of photography, the dynamic range is analogous to the contrast range....
Understanding Infinity-Corrected Objective Resolving Power and Magnification
This informative white paper from Edmund Optics covers a range of topics from the field of Microscopy, including terminology, application examples, and descriptions of different types filters such as dichroic, emission, and excitation filters used...
The Measurement of High Optical Densities (up to 8 Abs) in the Near-Infrared
The optical densities of various materials used in the manufacture of laser safety eyewear have been determined in the NIR. The lens materials were measured over wavelength ranges corresponding to the laser wavelengths for which the eyewear was...
Clearly Superior Epoxies for Optical Applications
As technology in the optics industry continues to advance and improve, careful consideration of the properties of epoxies must be evaluated. When determining the most effective adhesive to be used in opto-electronics, medical and other optic related...
Real-time Profiling for Focusing, M2, Divergence & Alignment
Beam intensity profiling is an essential tool in many aspects of photonics. The precise intensity distribution in a focused laser beam is critical in many applications: flow cytometry, laser printing, medical lasers, and cutting lasers are just a...
Dispersive 1064nm Raman Spectrometer Family
Owing to technological improvements spurred on by the telecommunications boom of the last decade, Raman spectroscopy has become much more accessible to users in all fields. The combination of improved technology and the technique’s molecular...
Super-resolution Microscopy
This article provides an overview of some of the key developments in super-resolution microscopy -- the art of enhancing the diffraction-limited resolution of an optical microscope.
The Benefits of Laser-Reflection Based Ultraprecision Active Alignment
Aligning and centering lenses for cementing or positioning in an optical system poses different challenges depending on the final precision requirements and the methods used during alignment. This paper details the benefits of laser-reflection-based...
An Introduction to FRET, with an Emphasis on the Optics Involved
Fluorescent Resonant Energy Transfer (FRET) is a process that involves two fluorochromes (molecules that have the potential to fluoresce) with overlapping emission and excitation spectra. FRET is useful in determining intra-molecular and...
Filter Sets for Imaging DsRed (Red Fluorescent Protein)
The new red fluorescent protein, DsRed, from Clontech can be viewed, and imaged, with a variety of filter sets due to its broad absorption and emission spectra. Using HeLa cells transfected with the DsRed protein, kindly provided by Clontech, we...
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April 2024
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