Microscopy | Photonics.com

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MICROSCOPY DICTIONARY TERMS

chemical microscopyThe field of microscopy as applied to chemical problems and analysis.

microscope immersion fluidThe liquid used in microscopy to fill the space between the high-power objective lens and the microscope slide in order to...

oblique illuminationA common technique in microscopy, oblique illumination is one in which the object is illuminated by a light source that is...

high-content screeningAlso known as HCS, an analytical method designed to collect statistically relevant amounts of quantitative data on many...

working distanceIn microscopy, the clear distance between the specimen being viewed and the first optical element of the objective lens.

ultraviolet microscopeA microscope used either to detect selective absorption of various wavelengths by the specimen or to achieve increased...

diffrimicroscopy

quantum cascade laserA Quantum Cascade Laser (QCL) is a type of semiconductor laser that emits light in the mid- to far-infrared portion of the...

fiber-based confocal luminescence microscopeA microscope in which laser light is delivered through single-mode fibers that replace the pinhole usually used in confocal...

microscopyThe technology of the interpretive use and applications of microscopes.

feret's diameterIn microscopy, the measured distance between theoretical parallel lines that are drawn tangent to the particle profile and...

holographic microscopyThe holographic recording of a microscopic specimen whereby magnification is achieved by alteration in a wavelength or...

vertical transmitted illuminationWith respect to microscopy, light that is directed through the specimen by a substage condenser.

cardioid condenserAn oil immersion condenser used to permit only light that has been diffracted or dispersed by a microscope specimen to enter...

magnetic lensAn arranged series of coils, magnets or electromagnets disposed in such a way that the resulting magnetic fields generate a...

Golgi Staining TechniqueThe Golgi staining technique (also called the black reaction) makes it possible to look at the nervous system by rendering...

optical stainingWith respect to microscopy, also called dispersive staining, optical staining the projection of colors through areas of a...

phase-contrast generationMicroscopy technique to convert the phase structure of the wave transmitted or reflected by the specimen into a...

morphological processingA set of algorithms used to study the structural and spatial characteristics of objects in a digitized image for...

interphako interference microscopyMeasures the refractive indices axially from the fiber profile. Microscopy technique provides an interferogram with high...

dark-field microscopyA technique whereby the sample is illuminated by a hollow cone of light larger than the acceptance angle of the objective,...

differential interference contrast microscopyDifferential interference contrast (DIC) microscopy, sometimes called Nomarski Microscopy after the inventor Georges...

Nomarski microscopyAlso referred to as differential interference contrast (DIC) microscopy; Nomarski microscopy is a unique form of microscopy...

photoelectron microscopySurface analysis by means of photon induced electron emission. PEM methods provide high lateral resolution of the observed...

video-intensified microscopy (VIM)The observation of specimens by a microscope linked to a television camera and an image processor.

point-projection x-ray microscopyA method of producing magnified images by x-rays. The specimen is placed close to a point source of x-rays; the...

two-photon excited fluorescenceTwo-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...

counting chamberIn microscopy, the chamber that is contained on a microscope slide to hold a certain amount of fluid. It is calibrated...

bright-field illuminationThe illumination generally used in microscopy, whereby the specimen appears dark against a light background.

zero-order filteringThe removal of the zero-order component of the Fourier spectrum distribution of an object with a small, opaque absorber or...

vertical incident illuminationWith respect to microscopy, light that is directed down through the objective onto the specimen and then returned by...

beta fluorographyThe use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials...

second-harmonic generation microscopyA nonlinear label-free imaging technique commonly used during surgical procedures for the visualization of collagen fibers...

X-ray Phase Contrast Microscopy (XRIM)Used for high-resolution surface study with subnanometer resolution. XRIM uses interfacial phase contrast with application...

carbon filmIn analysis, the carbon layer that is evaporation-deposited on a specimen to protect and ready it for study by electron...

laser microscopyTechnique using functional optical microscope with the addition of a coherent source collinear with the image path. The...

indirect illuminationThe light formed by visible radiation that, in traveling from light source to object, undergoes one or more reflections. In...

tip-enhanced Raman spectroscopyA spectroscopic technique that combines the high chemical specificity of Raman scattering and signal sensitivity provided by...

ultraviolet microscopyThe study and photographing of microscope specimens in ultraviolet light; using an optical microscope containing fluorite...

scanning coherent slope microscopyMeasures by heterodyning interferometry the local slope of a vibrating sample. The method allows the reconstruction of a...

stage micrometerIn microscopy, a calibrated scale on a slide that may be viewed to determine the exact magnification factor of the...

wide-field fluorescence microscopyWide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...

StereomicroscopyThe use of a specialized optical microscope designed to provide a more three-dimensional view of a sample. Stereomicroscopy...

direct illuminationLight produced by visible radiation that moves from the light source to the object without reflection. With respect to...

reflection x-ray microscopyA means of high resolution study through the application of soft and hard x-rays onto a sample surface in order to obtain...

epifluorescence microscopyFluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...

random access multiphoton microscopyAlso known as RAMP microscopy, random access multiphoton microscopy is a microscopic technique that uses multiple...

Light Sheet Fluorescence Microscopy (LSFM)Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and...

fluorescence microscopyObservation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane...

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