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Challenges and Opportunities in Superresolution Mar 28, 2017 — Much of our understanding of biology has come from our ability to image biological processes and structures through microscopes. But until recently, the imaging of intracellular structures and activities has been constrained by the optical resolution limit of the imaging systems themselves. This diffraction limit, first stipulated by Ernst Abbe in 1873, established the maximum resolution of an optical system to be about 200 nm in the X-Y (lateral) direction. (In addition, a theoretical limit of...
BioPhotonics, Jun 2017
Advances in Surgical Microscopes Pave the Way to Improved Outcomes Mar 2, 2017 — By integrating intelligence, video, intraoperative-imaging and navigation technologies, today’s surgical microscopes provide surgeons with insights to improve their decision-making at the point of care and provide patients with the best...
BioPhotonics, Apr 2017
Probing Cancer by Exploiting Spontaneous and Stimulated Raman Scattering Mar 1, 2017 — Most of our knowledge about the chemical contents in cancer cells or cancerous tissue is obtained from in vitro analysis of fixed cells and tissues or homogenates. These in vitro assays identify the presence of molecules, but do not provide...
BioPhotonics, Apr 2017
Modular Accessories Expand Fluorescence Microscope Capabilities Jan 25, 2017 — Despite the superior spatial resolution of complementary newer technologies such as atomic force microscopy (AFM), fluorescence microscopy remains a preeminent technology in the life sciences. Fluorescence detection offers a uniquely simple method...
BioPhotonics, Jan 2017
Superresolution Microscopy: An Imaging Revolution Jan 18, 2017 — Superresolution optical microscopy has been one of the most momentous developments in the life sciences over the last decade — and its evolution is ongoing. Progress has been so rapid and significant that it has been dubbed by some observers as “the...
Photonics.com, Mar 2017
Breaking the Speed Barrier for High-Fidelity, Large-Area Confocal Scanning Dec 16, 2016 — The need to image microstructures over very large areas is driving the next generation of instrumentation in microscopy. The challenge is complex: How can very large areas be scanned quickly and with high fidelity? Conventional wide-field...
BioPhotonics, Jan 2017
Fluorescence Correlation Spectroscopy — Going Beyond the Diffraction Limit Oct 11, 2016 — The combination of short measurement times along with free position or scanning of the observation spot makes fluorescence correlation spectroscopy an excellent tool for investigating diffusion heterogeneity over time and space.
BioPhotonics, Dec 2016
In Vitro Diagnostics, Immunology Spurring Advances in Flow Cytometry Oct 3, 2016 — Cells are the building blocks of organisms. From yeast and bacteria to elephants and whales, cells are the fundamental units of both biological structure and function. They have long been the subject of intense study: to better understand how they...
Photonics Spectra, Nov 2016
Nonlinear Microscopy Moves Into the Operating Room Sep 2, 2016 — The quality of a person’s vision can influence life-and-death situations, and this is especially true for a surgeon cutting with the scalpel. Consequently, over the years, researchers have developed several techniques to improve and enhance...
BioPhotonics, Oct 2016
Live-Cell Imaging Thrives With Microenvironment Control Systems Aug 4, 2016 — As microscopy techniques become more complex and require researchers to keep specimens under objectives for longer periods, microenvironmental control systems play an important role in pushing the limits of live-cell imaging.
BioPhotonics, Sep 2016
Holotomography Unlocks New Potential for Life Sciences Research Aug 4, 2016 — 3D holographic microscopy enables quantitative and noninvasive investigation of cells’ intrinsic properties — without labeling agents. Holotomography is now poised to advance disease diagnosis and treatment.
BioPhotonics, Sep 2016
Ensuring Accurate Quantitative Image Analysis Jul 18, 2016 — Quality control in materials inspection requires the ability to detect and quantify both minute surface imperfections, and those beneath the surface. Inspection is often conducted with digital imaging microscopy setups; however, such images are...
Photonics.com, Sep 2016
Tip-Enhanced Raman Spectroscopy Takes Bio Fingerprinting to Nanoscale Level Jul 15, 2016 — Despite being able to provide fingerprint information in biology, medicine and other fields, Raman spectroscopy suffers from a signal-to-noise ratio that is limited by the inherent weakness of Raman scattering and a spatial resolution that is...
BioPhotonics, Sep 2016
Innovation in Surface Tracking Opens Doors to Raman Imaging Applications Jul 14, 2016 — Scientists dream of being able to probe variations in the chemical composition and structure of materials without any form of sample preparation. Raman spectroscopy, a noncontact, nondestructive analysis tool that yields information on the chemical,...
Photonics Spectra, Sep 2016
When the Camera Is a Computer: Computational Life Sciences Imaging Jun 21, 2016 — Sensors and cameras continue to progress, although life sciences imaging remains far from perfect. Even in its current state, barriers still exist. These include signal photon noise, light scattering, optical blur of finite aperture imaging systems...
BioPhotonics, Aug 2016
High-Content Screening Goes Beyond Fluorescent Pictures Jun 20, 2016 — Since the start of high-content screening (HCS) in the late 1990s, several imaging techniques have been applied for automated image capture, serving a variety of application goals and specifications. However, technological advancements are enabling...
BioPhotonics, Aug 2016
New Quantum Dots Excite In Vivo Imaging Advancements Jun 17, 2016 — New quantum dots made without heavy metals are poised to take optical in vivo imaging to the next level.
BioPhotonics, Aug 2016
Neuroscience Driving Continued Growth in Multiphoton Microscopy Jun 15, 2016 — More than half of the thousands of deployed multiphoton microscopes are used for neuroscience — in large part because of major funding initiatives across three continents. Some of these initiatives are specifically targeted toward in vivo...
BioPhotonics, Aug 2016
Airy Beam Light-Sheet Microscopy Holds Promise for Life Sciences Imaging May 24, 2016 — Airy light-sheet microscopy has great potential to help further research and clinical diagnoses through its ability to image larger 3D volumes of tissues and organoids. Developed in the last three years, it is best-suited to the fields of...
Photonics Spectra, Jul 2016
Member Exclusive
Lighting Up Microscopes: Advances and Emerging Sources Apr 27, 2016 — Microscope developers are a resourceful bunch, opting to use the light source available to them at the time to peer at or below the surface of various materials. Even dating back to the 17th century, Galileo used sunlight to produce the very first...
Photonics Spectra, Jun 2016
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Optical Tweezers Bring Major Discoveries Within Grasp Feb 23, 2016 — Optical tweezers offer a noninvasive experimental method to trap, track and manipulate microscopic objects ranging from several tens of micrometers to tens of nanometers in size. The trapped particles can be accurately measured and manipulated with...
BioPhotonics, Apr 2016
Measuring Surface Roughness: The Benefits of Laser Confocal Microscopy Feb 10, 2016 — When evaluating the surface of a component, surface roughness can be assessed by eye or by rubbing it with a fingertip. Common expressions include “shiny,” “lusterless and rough,” “like oxidized silver,” or “like a mirror.” The differences indicated...
Photonics Handbook, Mar 2009
Nanopositioning: A Step Ahead Feb 1, 2016 — It is almost a cliché among students of creativity that innovation occurs at the intersection of fields. Since nanopositioning is a discipline that has enabled swiftly evolving application arenas as diverse as materials science, genomics, photonics,...
Photonics Handbook, Mar 2009
Microscopy Illumination: Considering Nonlaser Light Sources Jan 19, 2016 — As recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an arc source, typically 100 W mercury. Today, there...
Photonics Handbook, Mar 2009
Can Confocal Microscopes Compete with the Emergence of Superresolution? Jan 19, 2016 — Like most things, confocal microscopes have improved with age. The technique dates back to 1955, when American cognitive scientist Professor Marvin Minsky proposed that the ideal microscope would eliminate all light rays not initially aimed at the...
BioPhotonics, Jan 2016
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