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MICROSCOPY WHITE PAPERS
“Fingerprint” vs Handheld Raman Applications and the Different Optical Filters That Enable Them
Raman spectroscopy is a powerful non-invasive, non-contact tool capable of identifying molecular constituents of samples. Wavelength selective optical filters are used to block the dominant Rayleigh scattered light and transmit only the Raman photons providing “more signal, with less background” to the detector. It is critical to balance the performance and commercial needs of different markets and applications when selecting the optical filters for each application.
Is a DIY Microscope for You?
For some advanced microscopy and nanoscopy techniques it becomes necessary to modify existing instrumentation or build an instrument from scratch. This article helps readers determine whether a do-it-yourself (DIY) microscope is the appropriate...
Sub 50 Femtosecond Pulse Lasers for Gentler Multiphoton Microscopy
Finding the ideal ultra-fast laser source for a multiphoton imaging setup is not trivial. It is a fine balance between peak power, pulse energy and laser wavelength. In this white paper we show that the use of sub 50 fs pulsed lasers produces very...
FISBA READYBeam™ - Compact Powerful Laser Source
The FISBA READYBeam™ is our answer to customers' increasing demand for compact, powerful, and reliable multicolor laser source that can be easily integrated into existing set-ups. The multicolor laser module is available in four main configurations...
Innovating the Tunable Light Source - How the Tunable Laser System is Applied in Fluorescence Microscopy
The market for the tunable light source is ever expanding due to its uses in countless fields, such as the inspection industry, machine visioning, and a wide range of optical experiments. Spectrolight Inc. has recently announced the Tunable Laser...
Shanghai Optics Design for Manufacturing (DFM) Case Study
Shanghai Optics offers first-class Design for Manufacturing (DFM) services. We are proud of our track history helping customers improve product quality and reduce their manufacturing cost significantly. Below is one example of the optical lens we...
Principles of Scanning Nitrogen-Vacancy Magnetometry Explained
Scanning nitrogen-vacancy (NV) magnetometry is a measurement technique that combines scanned probe microscopy (SPM) with optically detected magnetic resonance (ODMR) to image magnetic field distribution with high spatial resolution (<50 nm). This...
How Right Angle Prisms Work?
How Right Angle Prisms Work? Mainly used in various optical instruments and optical imaging fields, for bending image paths or 90° turning rays. Right-angle prisms can produce left-handed images that are flipped or turned, depending on the...
Methods of Imaging with Photoelastic Modulators
This paper highlights a range of options to utilize Photoelastic Modulators (PEMs) with imaging systems to generate full field polarimetric measurements. Due to the resonant nature of the polarization modulation, the change in polarization cannot be...
Positioning at the Nanoscale (and Below): Trust in Proven Performance
Through a deep knowledge of sensing technologies, technically adept personnel, and experience across applications as diverse as astronomy to live cell imaging, Mad City Labs helps our customers develop cost-effective and high-performance solutions....
Cricket 2 Single MCP
Advanced Image Intensifier adapter for low light level imaging applications, the Cricket™² is a plug & play camera attachment enabling low light level imaging and extreme high shutter speeds for CMOS and CCD cameras. The Cricket™² fitted with a...
Product Development Strategies for Electro-Optical Systems
This whitepaper looks at the unique challenges associated with optical systems by exploring two extremes, an ultra-high precision instrument, and a low-cost consumer product, and the different approaches necessary for a successful product design and...
Ingenuity + Instrumentation: Creating a Novel 4D Microscopy Method
Tracking particles at the micro or nanoscale is inherently difficult due to the complexity of the systems studied, as well as the technical challenges: a blend of instrument design, photonics, modeling, and imaging. The Welsher Lab at Duke...
Precision Motion and How to Achieve It
Nanoscale motion requires fastidious attention to instrumentation, passive controls, and experiment design. Most users have experience in motion control within the sphere of the microscale. In this paper we discuss what needs to be considered in the...
Fluorescence, Delayed Fluorescence and Phosphorescence Spectra of a TADF Emitter Measured Using the FLS1000 with a VPL Laser and Gated PMT Detector
Materials exhibiting thermally activated delayed fluorescence (TADF) have attracted widespread research attention as a new generation of high-efficiency emitters for use in Organic Light Emitting Diodes (OLEDs). In a TADF OLED, triplet excitons are...
Photoluminescence Spectroscopy of Carbon Dots
Carbon dots (C-dots) are nanoparticles composed of carbon with diameters below 10 nm. They were accidentally discovered in 2004 by Xu, et al1 when researching methods to purify carbon nanotubes. Since their discovery they have received much...
Gemstone Identification Using Raman Microscopy
Raman microscopy is an ideal method for analysing gemstones and other geological samples. Its non-destructive nature and lack of sample preparation make it particularly advantageous for analysing all gemstones with no concerns about damage of...
Identification of Microplastics Using Raman Spectroscopy
This application note will investigate 3 polymers, polyethylene (PE), polytetrafluoroethylene (PTFE), and nylon-6, using the Edinburgh Instruments RM5 Raman Microscope.
Confocal Raman and Photoluminescence Microscopy for Forensic Investigations
Forensic investigations often study extremely small samples which may need analyses by multiple techniques. Confocal Raman and Photoluminescence (PL) Microscopy are well-suited techniques for dealing with such samples. This application note details...
Sb to Mn Energy Transfer Revealed using Time-Resolved Emission Spectroscopy (TRES)
Heterometallic metal halide hybrids hold great promise due to the potential for synergistic photo-physical properties depending on the choice of the constituent metal centres. In this application note time-resolved emission spectroscopy (TRES) with...
Optical Filters and LED Illumination
Understanding the interplay of optical filters, microscope light source and fluorophore selection can be a complex task, especially when it comes to multi-channel imaging. However, the selection of each component can make or break a fluorescence...
Solid-State Illumination for Multiplexed Fluorescence Detection
The capacity to simultaneously identify and localize multiple molecules or molecular assemblies in complex, heterogenous specimens has long been a primary attribute driving applications of fluorescence microscopy in the biological and physical...
Novel Hyperspectral Cameras for Low Light Applications
The white paper presents HERA, a novel hyperspectral camera, and a series of results obtained in different applications (agri-tech & food, plastic sorting, remote sensing, and microscopy). The HERA hyperspectral cameras, designed by NIREOS (Italy),...
Spatial Light Modulator 1024 x 1024
The 1024 x 1024 High-Speed Spatial Light Modulator is a beacon of advanced SLM technology and proprietary Meadowlark Optics software. The datasheet goes in-depth on its remarkable phase stability, diffraction efficiency, dielectric mirror coating,...
A Comparison of Some of the Key Technologies and Characteristics in CCD and CMOS Sensors
To assist in choosing the right technology for an application, this paper covers: • Historical background • Architecture • Improvements • Binning • Fill factor • Full well depth and capacity • Dynamic range • Frontside and backside illumination •...
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FEATURES
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