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MICROSCOPY WHITE PAPERS
Understanding Virus Mechanisms – One Particle at a TimeSingle-molecule microscopy techniques facilitate direct study of molecular mechanisms, enabling leaps in understanding surrounding how viruses assemble, disassemble, and interact with their hosts. In this researcher profile, we describe how Prof. Tijana Ivanovic is working to understand cell entry mechanisms and the relationship between virus particle structure/organization and the early steps of infection.
Knight Optical Meets Customer's Quality ExpectationsIt is with great pleasure that Knight Optical – the industry-leading supplier of metrology-tested, on-spec optical components – announces a 99.9% pass rate from its state-of-the-art Metrology Laboratory and dedicated QA department. Since 1991, we...
Recent Research Using Meadowlark Optics Spatial Light ModulatorsThis ever-evolving collection of recent research using Meadowlark Optics Spatial Light Modulators offers a brief overview of specific applications in which Meadowlark Optics’ Spatial Light Modulators are most commonly used. Citations to published...
Advantages of Measuring Surface Roughness with White Light InterferometryThis application note discusses the use of mean roughness measurements with white light interferometry (WLI) optical profilers. Spatial filters are explained, as well as some of the normative standards requirements from ASME B46.1-2009, ISO 13565-12...
Automation on a budget: Capturing high-speed multi-labelled events with LED illumination systemsThe light source for widefield fluorescence microscopy might seem like an unlikely enabler for high-speed live cell imaging, but the level of control that comes with LED Illumination Systems has changed the game. Capturing dynamic cellular processes...
Automated Scratch-Dig Inspection - Advantages of Replacing Manual-visual InspectionWorldwide a tendency can be observed to increase accuracy and quality of inspection of optical parts. More often than ever before, manufacturers explore computer-based inspection systems to gradually replace human inspectors. We describe how machine...
3 Lasers IN-ONE – FISBA READYBeam™ in Life SciencesThe FISBA READYBeam™ easily integrates into your set-up or instrument. Light-sheet Fluorescence Microscopy or Selective Plane Illumination (SPIM) enables high resolution imaging of large biological samples in three dimensions. Although the very...
3 Lasers-IN-ONE – Discover the FISBA READYBeam™!The development of diode emitters towards increasing power and variety, facilitated their usage in a rapidly growing field of scientific and industrial applications. In various cases it becomes possible to replace bulky gas or solid state lasers....
Light Engine Stability and LongevityOperational lifetimes of solid-state light sources ranging from 10,000–20,000 hours are often cited. These estimates are often derived for single light emitting diodes (LEDs) and are not representative of sophisticated, integrated light engines in...
Use of Single-Frequency DPSS Lasers in RamanWhilst diode lasers have advantages in terms of cost and efficiency, they are inherently limited in line-width narrowness and spectral purity. It’s particularly true for shorter wavelengths as it makes them unsuitable when high resolution is needed....
Is a DIY Microscope for you?Recently, it has become very popular for researchers to explore building their own microscopes. Budgetary and technical specifications are among the motivations for this approach. However, there is a spectrum of solutions within the DIY microscope...
Custom vs. Off-the-Shelf Solutions: Are you dreaming big enough?Custom positioning solutions provide more leeway for innovation, and at far less cost, than many customers realize. Irrespective of whether the goal is a one-off solution or multiple units for a wider industrial instrument, an evaluation of standard...
Are Large Image Sensors A Perfect Fit for Large Field of View Microscope Applications?In the last years nearly all microscope manufacturers used image circles with 18 mm diameter to image their field of view to cameras connected to their microscopes. Only recently some microscope manufacturers increased their field of view to offer...
Multi-Wavelength Laser Sources for Multi-Color Fluorescence MicroscopyMulti-color fluorescence microscopy is a widely used technique to generate multi-spectral images of cells and other small objects for identifying the spatial distribution of molecules of interest in complex heterogeneous samples. In this article, we...
Discovering Fine Neurovascular Structures in Tibial Epiphysis Using the FV3000 MicroscopeUnderstanding the vascular and neural projections to the knee joint is important for treating pain associated with knee arthropathy. With the FV3000 microscope, researchers at the Keio University School of Medicine were able to successfully image a...
Laser-Based Sample Preparation for Microstructure DiagnosticsThere is an increased demand for preparation techniques tuned to manifold methods of microstructure diagnostics that need to be fast, reliable, cost effective, artifact-free, and targeted. Traditional mechanical preparation and focused-ion-beam...
Close Is Not Close Enough: Micropositioners' Role In NanopositioningWhen working at the nanoscale, every input has seismic impact on the ability to view, position, or manipulate the object of their attention. Whether the application is in single-molecule spectroscopy, optical trapping, super-resolution microscopy,...
Fluorescence Microscopy Technique Provides New Views of Biological ProcessesIntroduction to the technique of MicroMirror TIRF (total internal reflection fluorescence) microscopy. The MicroMirror TIRF microscopy technique offers significant signal-to-noise ratio improvements compared to dichroic based TIRF microscopy when...
Twelve Reasons Why Your Next Confocal Should Be DragonflyAndor’s Dragonfly High Speed Confocal Platform offers outstanding performance and flexibility, so when your research calls for confocal, consider these twelve compelling reasons why Dragonfly should be next in your lab or core facility. Bottom-line,...
Fast Volumetric and Off-Angle Brain Imaging with the FVMPE-RS® Multiphoton MicroscopeMultiphoton microscopy has become the primary tool for in vivo studies of brain structure and activity at the cellular level in animal models ranging from small mice to larger mammals such as primates. As applications of this technique broaden,...
Camera-based Products - Benefits of an ISO13485:2016-Certified SupplierWhat advantages does a supplier certified according to the medical device standard ISO 13485:2016 offer to a manufacturer of camera-based medical products? Our White Paper describes the requirements of this standard, presents various ways in which...
A High-Quality Raman Microscope Can Be Recognized by These 5 CriteriaRaman spectroscopy long remained a technique that was only performed in special laboratories. In recent years, however, it has been increasingly losing its outsider status. The reason for this is the development of the confocal Raman microscope,...
Is It True That Cooled Cameras are More Sensitive than Non-cooled Cameras?The temperature has a significant impact on certain noise sources prevalent in cameras, which contribute to the total readout noise of an image sensor. When electronic devices are operated continuously, they dissipate heat due to losses in the...
Behind the Scenes of Today's Imaging ProcessImage processing has become so ingrained in everyday life that we often forget how quickly it has advanced over the last few decades. Today, we are able to snap photos and then rotate, crop, and apply various filters to the images directly on the...
Time-resolved Fluorescence Spectroscopy and Microscopy in Materials ScienceStudying luminescence lifetime data is a very powerful analytical tool to gain insights into the excited state dynamics of molecules, nanoparticles, or semiconductors. Time-resolved methods such as Fluorescence Lifetime Imaging are commonly used in...
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