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MICROSCOPY WHITE PAPERS
Light Engine Stability and LongevityOperational lifetimes of solid-state light sources ranging from 10,000–20,000 hours are often cited. These estimates are often derived for single light emitting diodes (LEDs) and are not representative of sophisticated, integrated light engines in typical working laboratory environments. This white paper provides operating lifetimes, cost considerations and stability data for Lumencor’s solid-state light engines based on service history data.
Optimising Irradiance for Fluorescence MicroscopyIn fluorescence microscopy, the light hitting the sample can make all the difference between an experimental breakthrough and inconclusive results. However, when optimising an experiment, components such as the light source can often be overlooked,...
Use of Single-Frequency DPSS Lasers in RamanWhilst diode lasers have advantages in terms of cost and efficiency, they are inherently limited in line-width narrowness and spectral purity. It’s particularly true for shorter wavelengths as it makes them unsuitable when high resolution is needed....
Is a DIY Microscope for you?Recently, it has become very popular for researchers to explore building their own microscopes. Budgetary and technical specifications are among the motivations for this approach. However, there is a spectrum of solutions within the DIY microscope...
Custom vs. Off-the-Shelf Solutions: Are you dreaming big enough?Custom positioning solutions provide more leeway for innovation, and at far less cost, than many customers realize. Irrespective of whether the goal is a one-off solution or multiple units for a wider industrial instrument, an evaluation of standard...
Are Large Image Sensors A Perfect Fit for Large Field of View Microscope Applications?In the last years nearly all microscope manufacturers used image circles with 18 mm diameter to image their field of view to cameras connected to their microscopes. Only recently some microscope manufacturers increased their field of view to offer...
Multi-Wavelength Laser Sources for Multi-Color Fluorescence MicroscopyMulti-color fluorescence microscopy is a widely used technique to generate multi-spectral images of cells and other small objects for identifying the spatial distribution of molecules of interest in complex heterogeneous samples. In this article, we...
Discovering Fine Neurovascular Structures in Tibial Epiphysis Using the FV3000 MicroscopeUnderstanding the vascular and neural projections to the knee joint is important for treating pain associated with knee arthropathy. With the FV3000 microscope, researchers at the Keio University School of Medicine were able to successfully image a...
Laser-Based Sample Preparation for Microstructure DiagnosticsThere is an increased demand for preparation techniques tuned to manifold methods of microstructure diagnostics that need to be fast, reliable, cost effective, artifact-free, and targeted. Traditional mechanical preparation and focused-ion-beam...
Close Is Not Close Enough: Micropositioners' Role In NanopositioningWhen working at the nanoscale, every input has seismic impact on the ability to view, position, or manipulate the object of their attention. Whether the application is in single-molecule spectroscopy, optical trapping, super-resolution microscopy,...
Fluorescence Microscopy Technique Provides New Views of Biological ProcessesIntroduction to the technique of MicroMirror TIRF (total internal reflection fluorescence) microscopy. The MicroMirror TIRF microscopy technique offers significant signal-to-noise ratio improvements compared to dichroic based TIRF microscopy when...
Twelve Reasons Why Your Next Confocal Should Be DragonflyAndor’s Dragonfly High Speed Confocal Platform offers outstanding performance and flexibility, so when your research calls for confocal, consider these twelve compelling reasons why Dragonfly should be next in your lab or core facility. Bottom-line,...
Fast Volumetric and Off-Angle Brain Imaging with the FVMPE-RS® Multiphoton MicroscopeMultiphoton microscopy has become the primary tool for in vivo studies of brain structure and activity at the cellular level in animal models ranging from small mice to larger mammals such as primates. As applications of this technique broaden,...
Camera-based Products - Benefits of an ISO13485:2016-Certified SupplierWhat advantages does a supplier certified according to the medical device standard ISO 13485:2016 offer to a manufacturer of camera-based medical products? Our White Paper describes the requirements of this standard, presents various ways in which...
A High-Quality Raman Microscope Can Be Recognized by These 5 CriteriaRaman spectroscopy long remained a technique that was only performed in special laboratories. In recent years, however, it has been increasingly losing its outsider status. The reason for this is the development of the confocal Raman microscope,...
Is It True That Cooled Cameras are More Sensitive than Non-cooled Cameras?The temperature has a significant impact on certain noise sources prevalent in cameras, which contribute to the total readout noise of an image sensor. When electronic devices are operated continuously, they dissipate heat due to losses in the...
Behind the Scenes of Today's Imaging ProcessImage processing has become so ingrained in everyday life that we often forget how quickly it has advanced over the last few decades. Today, we are able to snap photos and then rotate, crop, and apply various filters to the images directly on the...
Time-resolved Fluorescence Spectroscopy and Microscopy in Materials ScienceStudying luminescence lifetime data is a very powerful analytical tool to gain insights into the excited state dynamics of molecules, nanoparticles, or semiconductors. Time-resolved methods such as Fluorescence Lifetime Imaging are commonly used in...
Advantages of Optically Pumped Semiconductor Lasers – Invariant Beam PropertiesThe optically pumped semiconductor laser (OPSL) is a unique patented technology that combines the most desirable attributes of laser diodes, DPSS (Diode Pumped Solid State) and Ion lasers, while eliminating a number of their compromising...
High Performance Motion Control – Systems Approach Provides Nanometer Precision for Industrial ApplicationsHigh performance motion control plays a key part in positioning and handling applications, such as semiconductor manufacturing, laser and materials processing, optical inspection systems, additive manufacturing or industrial digital printing. In...
Hyperspectral Imaging Cameras in Rapid Detection and Potential Diagnosis of Agricultural Crop ThreatsRemote monitoring of crops via satellites using color cameras was the earliest form of spectral imaging in agricultural management. Due to limited capabilities, only basic plant health parameters could be reported. The advent of hyperspectral...
The VisIR-765 “STED” - A Versatile Picosecond Pulsed Laser Module for Spectroscopy and MicroscopyStudying luminescence lifetime is a very powerful analytical tool, as it provides insights into the excited state dynamics of molecules, complexes, or semi-conductors. Pulsed laser modules with temporal pulse widths in the range of tens to a few...
Embedded Motion Modules Make Great Products SmallerEmbedded motion modules are a new option for product design teams. Combining mechanical and electrical functions in one optimized, easy-to-integrate device, they speed the design process and ensure smallest system size. Electrical connections are DC...
Modern CMOS Sensors and Their Use in Fluorescence-Based ApplicationsDr. Felix Asche, Product Manager at Basler, outlines possibilities and application areas of modern CMOS sensor technology in medicine and science. System manufacturers whose devices are equipped with CCD sensors and who are therefore increasingly...
SRRF-Stream - Releasing The Potential of Real-Time Super-Resolution MicroscopySRRF-Stream super-resolution processing is carried at a rate up to 30x faster than the ImageJ implementation of SRRF, ‘NanoJ-SRRF’. Furthermore, SRRF-Stream processing is carried out in parallel with data acquisition, as opposed to being restricted...
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