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MICROSCOPY WHITE PAPERS
Resonant probe AFM: Uses and AdvantagesA resonant probe atomic force microscope (AFM) offers capabilities and benefits not found in commonly used optical deflection AFMs in the measurement of physical constants, physical properties, and surface morphology. This article discusses resonant probe AFM construction, functionality. Some of the applications particularly well suited to resonant probe AFM are optical antenna properties, quantum materials (NV center research), and terahertz microscopy/spectroscopy.
4 Steps to Optimize the Optics in Automated Imaging InstrumentsOptimize the performance and cost of your automated optical imaging system with 6 key equations for selecting an imaging sensor, objective lens, Z-focusing nano-positioning stage and XY sample positioning motion. Topics covered include: optimal...
Industrial Equipment Failure Analysis Using FT-IR Microscopic TechniquesThis work demonstrates that FT-IR microscopy analysis is capable of determining the cause of failures of many different pieces of industrial equipment. This analysis can then allow the user to take preventative action measures in order to improve...
Near-Field Scanning Optical Microscopes: Capabilities and ApplicationsNSOM techniques and instrumentation have evolved to become vital tools for material characterization, providing high-quality data and continually expanding utility, spot-on accuracy, adaptability, and exceptionally high definition. This article...
Atomic Force Microscopy Enhances the Nanoscopy ToolkitImaging and morphology represent a critical puzzle piece when trying to understand nanoscale structures, whether they are naturally occurring, such as viruses, or engineered structures, such as nano-antennas and photonic devices. In addition to...
Measuring and Correcting MicroLED Display UniformityAs individual emissive elements, microLEDs commonly exhibit luminance and color variations at the pixel level. These variations require each microLED to be measured and adjusted individually to ensure visual uniformity across the display. Thus, a...
Using Imaging Colorimeters to Correct OLED, MicroLED, and Other Emissive Displays for Production Efficiency and YieldsImaging colorimeters can improve OLED and microLED display production by ensuring visual quality of displays — measuring brightness and color uniformity at the pixel and subpixel level to correct poorly performing panels and safeguard manufacturing...
High-Speed Imaging in Biomedical Microfluidic ResearchThis whitepaper discusses multiple case studies when Phantom cameras have assisted in the advancement of microfluidic and cellular research.
Understanding Virus Mechanisms – One Particle at a TimeSingle-molecule microscopy techniques facilitate direct study of molecular mechanisms, enabling leaps in understanding surrounding how viruses assemble, disassemble, and interact with their hosts. In this researcher profile, we describe how Prof....
Knight Optical Meets Customer's Quality ExpectationsIt is with great pleasure that Knight Optical – the industry-leading supplier of metrology-tested, on-spec optical components – announces a 99.9% pass rate from its state-of-the-art Metrology Laboratory and dedicated QA department. Since 1991, we...
Recent Research Using Meadowlark Optics Spatial Light ModulatorsThis ever-evolving collection of recent research using Meadowlark Optics Spatial Light Modulators offers a brief overview of specific applications in which Meadowlark Optics’ Spatial Light Modulators are most commonly used. Citations to published...
Advantages of Measuring Surface Roughness with White Light InterferometryThis application note discusses the use of mean roughness measurements with white light interferometry (WLI) optical profilers. Spatial filters are explained, as well as some of the normative standards requirements from ASME B46.1-2009, ISO 13565-12...
Automation on a budget: Capturing high-speed multi-labelled events with LED illumination systemsThe light source for widefield fluorescence microscopy might seem like an unlikely enabler for high-speed live cell imaging, but the level of control that comes with LED Illumination Systems has changed the game. Capturing dynamic cellular processes...
Automated Scratch-Dig Inspection - Advantages of Replacing Manual-visual InspectionWorldwide a tendency can be observed to increase accuracy and quality of inspection of optical parts. More often than ever before, manufacturers explore computer-based inspection systems to gradually replace human inspectors. We describe how machine...
3 Lasers IN-ONE – FISBA READYBeam™ in Life SciencesThe FISBA READYBeam™ easily integrates into your set-up or instrument. Light-sheet Fluorescence Microscopy or Selective Plane Illumination (SPIM) enables high resolution imaging of large biological samples in three dimensions. Although the very...
3 Lasers-IN-ONE – Discover the FISBA READYBeam™!The development of diode emitters towards increasing power and variety, facilitated their usage in a rapidly growing field of scientific and industrial applications. In various cases it becomes possible to replace bulky gas or solid state lasers....
Light Engine Stability and LongevityOperational lifetimes of solid-state light sources ranging from 10,000–20,000 hours are often cited. These estimates are often derived for single light emitting diodes (LEDs) and are not representative of sophisticated, integrated light engines in...
Is a DIY Microscope for you?Recently, it has become very popular for researchers to explore building their own microscopes. Budgetary and technical specifications are among the motivations for this approach. However, there is a spectrum of solutions within the DIY microscope...
Custom vs. Off-the-Shelf Solutions: Are you dreaming big enough?Custom positioning solutions provide more leeway for innovation, and at far less cost, than many customers realize. Irrespective of whether the goal is a one-off solution or multiple units for a wider industrial instrument, an evaluation of standard...
Are Large Image Sensors A Perfect Fit for Large Field of View Microscope Applications?In the last years nearly all microscope manufacturers used image circles with 18 mm diameter to image their field of view to cameras connected to their microscopes. Only recently some microscope manufacturers increased their field of view to offer...
Multi-Wavelength Laser Sources for Multi-Color Fluorescence MicroscopyMulti-color fluorescence microscopy is a widely used technique to generate multi-spectral images of cells and other small objects for identifying the spatial distribution of molecules of interest in complex heterogeneous samples. In this article, we...
Discovering Fine Neurovascular Structures in Tibial Epiphysis Using the FV3000 MicroscopeUnderstanding the vascular and neural projections to the knee joint is important for treating pain associated with knee arthropathy. With the FV3000 microscope, researchers at the Keio University School of Medicine were able to successfully image a...
Laser-Based Sample Preparation for Microstructure DiagnosticsThere is an increased demand for preparation techniques tuned to manifold methods of microstructure diagnostics that need to be fast, reliable, cost effective, artifact-free, and targeted. Traditional mechanical preparation and focused-ion-beam...
Close Is Not Close Enough: Micropositioners' Role In NanopositioningWhen working at the nanoscale, every input has seismic impact on the ability to view, position, or manipulate the object of their attention. Whether the application is in single-molecule spectroscopy, optical trapping, super-resolution microscopy,...
Fluorescence Microscopy Technique Provides New Views of Biological ProcessesIntroduction to the technique of MicroMirror TIRF (total internal reflection fluorescence) microscopy. The MicroMirror TIRF microscopy technique offers significant signal-to-noise ratio improvements compared to dichroic based TIRF microscopy when...
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Lasers and Their Uses Reference Wall Chart
Part of Photonics Media's celebration of the 50th anniversary of the laser, this chart lists the...
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