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fluorescence microscopy Dictionary Terms

autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when exposed to light. Unlike fluorescence that results from the application of external fluorophores or dyes, autofluorescence...
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...
fluorescent protein
Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a...
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...
microscopy
Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...
long-pass filter
A long-pass filter is an optical filter that allows longer wavelengths of light to pass through while blocking or...
scientific complementary metal-oxide semiconductor
Scientific complementary metal-oxide semiconductor (sCMOS) refers to a type of image sensor technology specifically designed...
optical filter
An optical filter is a device that selectively transmits or blocks specific wavelengths, or colors, of light. Optical...
intensified charge-coupled device camera
An intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an...
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
two-photon excited fluorescence
Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...
ultraviolet fiber optics
Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet...
photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...
epifluorescence microscopy
Fluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
light sheet fluorescence microscopy
Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and...
nanoparticle photonic resonator absorption microscopy
Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...
blue diode laser
A blue diode laser is a type of semiconductor laser that emits light in the blue wavelength range of the electromagnetic...
calcium indicator
A calcium indicator is a molecule that is sensitive to changes in calcium ion (Ca2+) concentrations in biological systems....
wide-field fluorescence microscopy
Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
electron multiplication charge-coupled device camera
An EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
dichroic mirror
A dichroic mirror, also known as a dichroic beamsplitter or interference filter beamsplitter, is an optical device that...

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