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fluorophore Dictionary Terms

fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
stochastic optical reconstruction microscopy
Stochastic optical reconstruction microscopy (STORM) is a super-resolution microscopy technique that enables imaging of...
single-molecule FRET
Single-molecule Förster resonance energy transfer (smFRET) is a specialized technique in biophysics and molecular...
time-correlated single photon counting
Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
superresolution
Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...
autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a super-resolution imaging technique in fluorescence...
single molecule localization microscopy
Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
single-photon avalanche diode
A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...

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