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Getting Outstanding Results From Multispectral TCSPC Fluorescence Lifetime Imaging

Oct 26, 2022
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Heitz discusses how scientific results from various cutting-edge studies performed by Becker & Hickl and the company’s collaborators were generated and what to consider when planning fluorescence lifetime imaging (FLIM) experiments. Data from both automatic laser-scanning experiments and a novel hand-held laser-scanning technique will be presented. Accurately determining the fluorescence lifetimes of a sample using time-correlated single-photon counting (TCSPC) greatly enhances the capabilities of conventional fluorescence imaging techniques. The fluorescence lifetime provides direct insight into the interaction of the fluorophore with the local environment. Mapping the fluorescence lifetimes across a sample surface produces a fluorescence lifetime image, combining the lifetime information with the sample structure. The information content of such a data set is further enhanced when multiple excitation wavelengths, detection channels, and wide lifetime ranges (from a few nanoseconds to seconds) are measured at the same time. Applications for this technique range from the life sciences and medicine to solid-state physics and engineering. Starting with the basic principles of FLIM, Heitz explores the multitude of capabilities this well-developed technique offers today.

***This presentation premiered during the 2022 BioPhotonics Conference. For more information on Photonics Media conferences, visit  

About the presenter
Julius HeitzJulius Heitz, Ph.D., studied physics at Heriot-Watt University and received a doctorate from the Free University of Berlin and the Fritz Haber Institute of the Max Planck Society. For his doctorate, Heitz studied spintronic effects in solid-state samples with high-field terahertz radiation sources, gaining a strong basis in nonlinear optics and femtosecond laser physics along the way. He is currently an applications specialist at Becker & Hickl GmbH, with a focus on R&D and customer support, working with time-correlated single-photon counting (TCSPC). In his free time, he rides motorcycles and enjoys the outdoors.
ImagingflourescenceBiophotonicsFLIMfluorescence lifetime imaging
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