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Fluorescence Imaging for Microbial Keratitis and Other Diseases of the Anterior Segment

Oct 26, 2023
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About This Webinar
Microbiological culture remains the primary tool for diagnosis of cornea infections such as microbial keratitis (MK) despite the 50% median positivity rate, and the reality that clinical impression and history alone are insufficient for diagnosis. Similarly, slit-lamp biomicroscopy remains the most common imaging tool for assessment of the anterior segment, but results are subjective and require expert training for interpretation. Improved diagnostic technologies for MK and other anterior segment diseases are needed. In this presentation, two-color fluorescence imaging is proposed as a method to diagnose MK and investigate other anterior segment diseases.

Herzog presents a detailed discussion of the imaging method, ocular and microbial fluorescence, and radiative transfer. He shares sample imaging and spectroscopy data that demonstrates the utility of the technique, and simulation results to aid in understanding the imaging technique. Herzog’s results suggest that fluorescence imaging is a promising tool for rapid, low-cost screening of MK and potentially other anterior segment diseases.

*** This presentation premiered during the 2023 BioPhotonics Conference. For more information on Photonics Media conferences and summits, visit events.photonics.com.

About the presenter

Joshua HerzogJoshua M. Herzog, Ph.D., earned his doctorate in nuclear engineering & engineering physics from the University of Wisconsin-Madison in 2020 focusing on quantitative laser diagnostics for turbulent, reacting flows. Since then, he has worked as a postdoctoral research fellow at the University of Michigan (UM) where his current work aims to develop novel imaging diagnostics for biomedical and health science applications, with a significant focus on investigating diseases of the anterior segment of the eye.

In addition, Herzog collaborates with several other groups at UM to develop and apply quantitative optical techniques for fundamental energy transfer and fluid mechanics studies. The primary goal of his research is to develop a detailed physical understanding of the light-matter interaction that takes place in optical and imaging measurements to enable more quantitative analysis. His additional research interests include fundamental atomic, molecular, and optical physics and computational optics.
ImagingBiophotonicsanterior segmentfluorescence imaging
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