Multiphoton Imaging in Neuroscience

Oct 26, 2022
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Sponsored by
Prior Scientific Inc.
TOPTICA Photonics Inc.
About This Webinar
Multiphoton imaging in neuroscience has much evolved from the initial two-photon microscope that Winfried Denk built in Watt Webb’s lab at Cornell University in the late 1980s, which made the live imaging of cellular interactions possible. Mastron discusses developments in multiphoton imaging in neuroscience, including contributions from disciplines such as biochemistry, signal processing, optics, and optoelectronics, and culminating in a discussion of current uses of in vivo multiphoton calcium imaging measurements in freely moving mice. He introduces facets of multiphoton imaging to a nonspecialist audience (genetically encoded calcium indicators, laser requirements for 2PEF, scanning systems, and detectors) and provides an overview of some technical advancements in the field for a specialist audience (multiplexed detection schema, adaptive optics, fiber laser technology, and Mini2P microscopes).

***This presentation premiered during the 2022 BioPhotonics Conference. For more information on Photonics Media conferences, visit  

About the presenter
Joseph MastronJoseph Mastron, Ph.D., joined TOPTICA Photonics Inc. as the ultrafast applications scientist in January 2022. He began working with ultrafast lasers in 2011 during an undergraduate solar energy research internship. While working in Steve Bradforth’s group at the University of Southern California, Mastron studied energy transfer and singlet fission in amorphous organic photovoltaic systems using optical pump-probe measurements.

In 2013, he began his doctorate studies in the Department of Chemistry at the University of Chicago. There, Mastron developed two-photon-excited and time-domain Fourier transform fluorescence-encoded infrared spectroscopic techniques in the lab of Andrei Tokmakoff and earned his doctorate in physical chemistry in 2019. In addition, he served with the University of Chicago Graduate Council as chair of marketing operations. From there, Mastron joined the Ogilvie and Kubarych groups at the University of Michigan as a postdoctoral research fellow joint between the departments of physics and chemistry, in the Laboratory for Ultrafast Multidimensional Optical Spectroscopy. He worked with multidimensional pump-probe and 2D techniques spanning the UV, visible, and IR wavelength ranges on a variety of systems, including inorganic catalysts, vibrational polaritons, and photosynthetic proteins. Additionally, he served University of Michigan postdocs as the chair of ethics, co-chair of PR, and co-president of the University of Michigan Postdoctoral Association of the College of Engineering.
Imagingultrafast lasersBiophotonicsmultiphoton imagingneuroscienceMicroscopy
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