Time-Lapse Imaging with Lamp and LED – Stability vs Repeatability

Nov 5, 2014
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Join us for a FREE webinar: Wednesday, November 5, 2014, 2pm EST

Fluorescence microscopy is a standard technique used in most laboratories around the world. With the invention of fluorescence proteins such as GFP, publications using fluorescence techniques have skyrocketed. Traditional HBO/mercury lamps were replaced by pre-aligned lamps that emerged in the early 2000s. With their broad spectrum and high output, these lamps have provided an effective solution for many years. However, mercury lamp solutions have drawbacks such as limited bulb lifespans, maintenance costs and warm-up time. These limitations can be eliminated by a newer technology - light emitting diode (LED) solutions

Until recent years, LEDs have been a technology faced with a number of barriers that have prevented wide commercial adoption. High cost and limited availability of LEDs, as well as low output and efficiency, were factors that prohibited market adoption With advancements in LED technology today, utilization of LEDs for fluorescence microscopy illumination has become increasingly popular in the industry. There are advantages that LEDs bring to microscopy applications.

This webinar will discuss the differences in stability between Lamps and LEDs, and also explain the importance of repeatability when comparing images taken between time points, whether this is within the same short experiment or a longer time-lapse experiment

Dr. Kavita Aswani is the Senior Applications Scientist for the Life Sciences products at Excelitas Technologies. She holds a Ph.D. from the University of Iowa and has 7 years of scientific research experience and over 14 years of applications experience in the microscopy and fluorescence industry. Kavita has authored and reviewed several publications as well as journal articles. She has worked in the areas of widefield illumination microscopy, laser scanning microscopy and flow cytometry. She is an active member of the Society for Neuroscience and the Royal Microscopical Society.
time-lapse imagingfluorescence microscopyMicroscopyExcelitas TechnologiesBiophotonicsLight SourcesImagingLEDs
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