Search Menu
Photonics Media Photonics Buyers' Guide Photonics EDU Photonics Spectra BioPhotonics EuroPhotonics Industrial Photonics Photonics Showcase Photonics ProdSpec Photonics Handbook
More News
Email Facebook Twitter Google+ LinkedIn

An Introduction to FRET, with an Emphasis on the Optics Involved

Monday, January 18, 2010
Fluorescent Resonant Energy Transfer (FRET) is a process that involves two fluorochromes (molecules that have the potential to fluoresce) with overlapping emission and excitation spectra. FRET is useful in determining intra-molecular and intermolecular reactions both spatially and temporally. It can be used to follow and record the interactions of proteins, enzymes, DNA, and RNA well below the 200 nanometer (10-9 meter) resolution limit of the optical microscope at its highest magnification and with the highest numerical aperture (NA) optics. In fact, FRET only allows resolution of interactions between 1 and 5nm, which is its beauty as well as its curse.
File: An_Introduction_to_FRET,_with_an_Emphasis_on_the_Optics_Involved.pdf (2.30 MB)
To download this white paper, please complete the *required fields before clicking the "Download" button.
Your contact information
* First Name:
* Last Name:
* Email Address:
* Company:
Address 2:
Postal Code:
* Country:
Phone #:
Fax #:

Register or login to auto-populate this form:
Login Register
* Required

By clicking 'Download', you are indicating that you have read and agree with our
Terms and Conditions and Privacy Policy.
acceptorautofluorescenceBiophotonicsC. Michael StanleyCFPChroma Technology Corp.cyan fluorescent proteindichroic mirrorsDNAdonoremission filtersemission spectraenzymesexcitation filtersfluorescent resonant energy transferfluorochromesFRETnoisenonradiative phenomenonopticsproteinsRNAwidefield microscopeyellow fluorescent proteinYFP
Terms & Conditions Privacy Policy About Us Contact Us
back to top

Facebook Twitter Instagram LinkedIn YouTube RSS
©2018 Photonics Media
x We deliver – right to your inbox. Subscribe FREE to our newsletters.