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Imaging Cells in Flow

Wednesday, August 20, 2014
Fluorescence microscopy enables the study of cells with molecular specificity and subcellular resolution. Despite recent improvements in the brightness of fluorescent labels and in the sensitivity of image sensors, imaging is usually limited to stationary cells. Imaging fluorescence in moving cells is fundamentally challenging because the exposure time is constrained by motion-blur, which limits the available signal. Ethan Schonbrun and his colleagues at Harvard University have developed a method that enables fluorescence imaging of fluorescently labelled cells traveling at high linear velocities in fluids.
File: Andor_Learning_Imaging_Cells_in_Flow.pdf (261.97 KB)
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BiophotonicsimagingSensors & DetectorsAndor Technologymolecular specificitysubcellular resolutionfluorescence microscopyimaging fluorescenceEthan Schonbrunflowing cellsAndor Neo scientific cmos camerapseudo-random excitation pulse sequence
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