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Use of White Light Fluorescence LED in long-term Live Cell Imaging Applications

Author: Beate Neumann, Yury Belyaev and Kavita Aswani
Thursday, September 04, 2014
Lamps have long been used in microscopy facilities to conduct time-lapse imaging experiments. Light uniformity, stability and sample exposure are key in generating reliable data. In this study, we evaluate an LED system (X-Cite® 120LED) for our high-content time-lapse experiments and show that LEDs provide a better solution for imaging live cells when compared with traditional lamps. The results illustrate that LEDs provide higher stability and better uniformity for imaging. In addition, LEDs allow for higher cell proliferation when compared with a traditional HBO lamp.
File: X_Cite_EMBL_Application_Note_FINAL_WEB_12_2013.pdf (1.41 MB)
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imagingBiophotonicsMicroscopylight sourcesLEDsmercury lamplive cell imaginglight stabilityproliferationLumen Dynamics
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