Search Menu
Photonics Media Photonics Buyers' Guide Photonics Spectra BioPhotonics EuroPhotonics Vision Spectra Photonics Showcase Photonics ProdSpec Photonics Handbook
More News

Measuring Illumination Intensity with Accuracy and Precision

Facebook Twitter LinkedIn Email Comments
Wednesday, November 06, 2019
CoolLED Ltd.

Since LED illumination systems for fluorescence microscopy arrived on the market, what is often known as "illumination intensity" has been a hot topic. The amount of light reaching the sample significantly impacts the brightness and contrast of fluorescence microscopy images and therefore data quality. However, the topic has been surrounded by complex, confusing, and sometimes inaccurate measurements and terminology. Even the term "intensity" is inaccurate. A key issue has been an industry-wide lack of a standardised approach to measure illumination, and in our new white paper we are setting out to change this. We introduce a protocol whereby microscopists can quantify the amount of light at the sample plane and therefore directly compare systems to find their ideal solution.

File: Measuring_illumination_intensity.pdf (386.04 KB)
To download this white paper, please complete the *required fields before clicking the "Download" button.
Your contact information
* First Name:
* Last Name:
* Email Address:
* Company:
Address 2:
Postal Code:
* Country:
Phone #:
Fax #:

Register or login to auto-populate this form:
Login Register
* Required

When you click "Download", you agree that your personal contact information may be shared with CoolLED Ltd. and they may contact you about their products and services in the future. You also agree that Photonics Media may contact you with information related to this request, and that you have read and accept our Privacy Policy and Terms and Conditions of Use.
Biophotonicslight sourcesMicroscopyintensityirradiance
back to top
Facebook Twitter Instagram LinkedIn YouTube RSS
©2019 Photonics Media, 100 West St., Pittsfield, MA, 01201 USA,

Photonics Media, Laurin Publishing
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.