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Measuring Illumination Intensity with Accuracy and Precision

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Wednesday, November 06, 2019
CoolLED Ltd.

Since LED illumination systems for fluorescence microscopy arrived on the market, what is often known as "illumination intensity" has been a hot topic. The amount of light reaching the sample significantly impacts the brightness and contrast of fluorescence microscopy images and therefore data quality. However, the topic has been surrounded by complex, confusing, and sometimes inaccurate measurements and terminology. Even the term "intensity" is inaccurate. A key issue has been an industry-wide lack of a standardised approach to measure illumination, and in our new white paper we are setting out to change this. We introduce a protocol whereby microscopists can quantify the amount of light at the sample plane and therefore directly compare systems to find their ideal solution.

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File: Measuring_illumination_intensity.pdf (386.04 KB)
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