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Time-Lapse Imaging with Lamp and LED – Stability vs Repeatability
Author: Kavita Aswani PhD, Senior Applications Scientist, Excelitas
Monday, November 17, 2014
Excelitas Technologies Corp.
Advancements in LED technology today enable LEDs to provide a compelling alternative to lamps for fluorescence microscopy illumination and have become increasingly popular. This article discusses the differences in stability between Lamps and LEDs, and explains the importance of repeatability versus stability when comparing images taken between time points, whether this is within the same short experiment or a longer time-lapse experiment.
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Lamps have long been used in microscopy facilities to conduct time-lapse imaging experiments. Light uniformity, stability and sample exposure are key in generating reliable data. In this study, we...
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