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FRET FILTERS

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Omega-Optical.jpgAvailable from Omega Optical is the FRET technical application note “Optimizing Filter Sets for FRET Applications.” Downloadable from the company Web site, the four-page document begins with an overview of the Förster resonance energy transfer phenomenon. In a discussion on filters and microscope configurations, it notes that two emission filters are required for the process — for the acceptor and for the donor fluorophore. Multiview attachments that produce a simultaneous split-screen view of the sample are covered, along with automated emission filter wheels and separate filter cubes.
PI Physik Instrumente - Revolution In Photonics Align ROS MR 3/24


Published: July 2006
Glossary
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external energy source, such as ultraviolet or visible light. The process by which a fluorophore absorbs and then re-emits light is known as fluorescence. Fluorophores are widely used in various scientific and technological applications, including fluorescence microscopy, flow cytometry, medical imaging, and molecular biology. Key features of fluorophores include: Excitation and...
BreakthroughMediafluorophoreFRET applicationsMicroscopyNew MediaOmega Optical

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