Search
Menu
Gentec Electro-Optics Inc   - Measure With Gentec Accuracy LB

'FlowCytobot' Detects Blooms

Facebook X LinkedIn Email
The Imaging FlowCytobot, an underwater microscope developed as a research tool, recently proved its worth as an early warning device by detecting blooms of harmful marine algae in the Gulf of Mexico and giving authorities time to close areas before people had eaten tainted shellfish.

Working with Rob Olson and Heidi Sosik -- plankton biologists and instrument developers at Woods Hole Oceanographic Institution (WHOI) in Falmouth -- biological oceanographer Lisa Campbell of Texas A&M University used the Imaging FlowCytobot instrument to detect a substantial increase in the abundance of the algae Dinophysis acuminata in the waters of Port Aransas, Texas, which led authorities to close shellfish beds in parts of the state for a month.
RobOlsonHeidi-Sosik.jpg
Woods Hole Oceanographic Institution biologists Rob Olson and Heidi Sosik examine plankton-filled water samples on a prototype of the Imaging FlowCytobot in Olson's laboratory. (Photo courtesy Tom Kleindinst, WHOI)
Dinophysis acuminata produces okadaic acid, a toxin that accumulates in shellfish tissues and can cause diarrhetic shellfish poisoning (DSP) in humans. DSP is not life-threatening but extremely unpleasant, with symptoms including nausea, cramping, vomiting, and diarrhea. Cooking does not destroy the toxin in the shellfish.

The Imaging FlowCytobot -- an automated, submersible cell analyzer developed at WHOI -- counts microscopic plants in the water and photographs them. The images and data are relayed back to a shore-based laboratory, where specially developed software automatically classifies the plankton into taxonomic groups.

“It is very satisfying to find that a technology we developed as a research tool can be so effective for protecting human health,” said Olson, who has worked with Sosik for several years to prototype and modify flow cytometers, which are more typically used in many biological and medical laboratories.

“We designed the Imaging FlowCytobot for continuous monitoring of a wide range of plankton, and that turns out to be just what was needed to detect a harmful algal bloom that no one expected.”

The discovery of the Dinophysis bloom came while the researchers were actually looking for something else. Campbell, Olson, Sosik, and colleagues deployed the instrument in the fall of 2007 at the University of Texas Marine Sciences Institute laboratory in the Mission Bay Aransas National Estuarine Research Reserve.

Their principal goal was to observe Karenia brevis, another toxic alga that blooms periodically in the Gulf and can lead to neurotoxic shellfish poisoning. The research team would like to observe the next K. brevis bloom before it happens; such blooms are most common and most extreme in the Gulf of Mexico in the late summer and fall.
toxicalga.jpg
The Imaging FlowCytobot captured this image of the toxic alga Dinophysis acuminata during the bloom in March 2008. (Photo courtesy Heidi Sosik and Rob Olson, WHOI, and Lisa Campbell, Texas A&M)
The team is also working to catalog the types and relative abundances of marine plants in the area throughout the year.

Cognex Corp. - Smart Sensor 3-24 GIF MR

In mid-February, Campbell reviewed plankton images collected by the Imaging FlowCytobot and detected a substantial increase in the abundance of the dinoflagellate Dinophysis, which occurs naturally in ocean waters worldwide but not usually in harmful quantities.

“We have never before observed a bloom of Dinophysis acuminata at such levels in the Gulf of Mexico,” Campbell said.

After reporting the increase to fellow researchers in coastal Texas, Campbell and colleagues collected water samples to confirm that algal toxins were present in the water. Other researchers collected oyster samples and sent them for toxin analysis at a US Food and Drug Administration laboratory.

On March 8 the Texas Department of State Health Services closed Aransas, Corpus Christi, and Copano bays to shellfish harvesting and recalled Texas oysters, clams, and mussels that had been sold between March 1-7.

A week later, six other bays and estuaries along the coast were closed. As of April 11, most shellfishing areas had reopened.
FlowCytobot.jpg
Researchers (l-r) Rob Olson, Marga McElroy, Alex Shalapyonok, and Heidi Sosik lower the FlowCytobot onto the WHOI research vessel Mytilus for deployment at the Martha's Vineyard Coastal Observatory. (Photo courtesy Tom Kleindinst, WHOI)
The bloom and subsequent warnings occurred just days before the Fulton Oysterfest, a major shellfish festival in the region. At last report, no shellfish-related human illnesses have been reported in Texas this spring.

“This is exactly what an early warning system should be,” said Campbell. “It should detect a bloom before people get sick. So often, we don’t figure out that there is a bloom until people are ill, which is too late. The Imaging FlowCytobot has proven itself effective for providing an early warning.”

“With time, we have come to see that the instrument has obvious practical uses,” said Sosik. “It now appears ready to make the transition from basic research tool to operative tool."

Funding for Campbell’s monitoring program and construction of the instrument was provided by the National Oceanic and Atmospheric Administration’s Cooperative Institute for Coastal and Estuarine Environmental Technology.

Funding for instrument development and earlier prototypes of the FlowCytobot and the Imaging Flow Cytobot was provided by WHOI through its Ocean Life Institute, Coastal Ocean Institute, Bigelow Chair, and Access to the Sea Fund, and by the National Science Foundation.

For more information, visit: www.whoi.edu

Published: April 2008
Glossary
photonics
The technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. The science includes light emission, transmission, deflection, amplification and detection by optical components and instruments, lasers and other light sources, fiber optics, electro-optical instrumentation, related hardware and electronics, and sophisticated systems. The range of applications of photonics extends from energy generation to detection to communications and...
algaebiologicalBiophotonicsbloomcell analyserDinophysis acuminataestuariesflow cytometerfood and drug administrationImaging FlowCytobotk. brevismarinemedicalMicroscopyNews & FeaturesoceanOysterfestoystersphotonicsplanktonshellfishtaintedTexastoxinunderwaterUniversity of TexasWatersWHOIWoods Hole Oceanographic Institution

We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.