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Jan 2007
An addition to the “Methods Express” series, the research guide Immunohistochemistry describes emerging techniques and more established methods, encompassing trends in automation and high throughput, and an expanding range of applications. Chapters discuss the benefits and disadvantages of various approaches, while providing detailed protocols and practical advice for use at the bench. Suitable for use by new and experienced researchers, the 250-page laboratory manual includes a full-color section and addresses antibodies, confocal microscopy, quality assurance, the selection of reporter labels, immunochemical staining techniques, ultrastructural and automated immunochemistry, and image capture, analysis and quantification. Simon Renshaw, ed.; Scion Publishing Ltd., Bloxham, UK, 2006; $79, paperbound; $137, hardbound.

Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by using labeled antibodies to bind specifically to their antigens. IHC is used for disease diagnosis, biological research and in drug development. Using IHC visualized tumor markers such as enzymes, oncogenes and tumor-specific antigens, physicians can diagnose if a tumor is benign or malignant and determine the stage and grade of a cancer.
ApplicationsBasic ScienceBiophotonicsBreakthroughMediaimmunohistochemistryMicroscopyNew MediaScion Publishing Ltd.

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