Search
Menu
Cognex Corp. - Smart Sensor 3-24 GIF LB

Spinning Disk vs. Laser-Scanning Confocal Microscopes

Facebook X LinkedIn Email
Nicolas George, Olympus America Inc.

When imaging thick biological specimens, light from out-of-focus planes often obscures the fluorescence of structures of interest. Various confocal methods can remove out-of-focus light to reveal the structures. Different confocal systems allow biologists to balance two sometimes incompatible priorities — rejecting as much unwanted light as possible while maintaining a detectable signal and perhaps a viable specimen. In laser scanning confocal systems, point laser excitation and the emission pinhole combine to remove most out-of-focus light. The excitation laser source scans across the...Read full article

Related content from Photonics Media



    Articles


    Products


    Photonics Handbook Articles


    White Papers


    Webinars


    Photonics Dictionary Terms


    Media


    Photonics Buyers' Guide Categories


    Companies
    Published: October 2004
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    Featuresfluorescenceimaging thick biological specimenslaser scanning confocal systemsMicroscopySensors & Detectors

    We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.