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FRET Technique Developed to Observe DNA-Repairing Protein

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CHAMPAIGN, Ill., Aug. 15, 2006 -- Researchers have observed the life cycle of RecA, a protein that plays a major role in repairing damaged DNA, by using a highly sensitive, single-molecule fluorescence resonance energy transfer (FRET) technique they developed . A better understanding of how these proteins function could help scientists' better understand mutations that form cancer. RecA is a DNA recombination protein found in the gut bacterium E. coli. A human homolog, called Rad51, interacts with many proteins, including BRCA2, whose mutation increases susceptibility to breast and ovarian cancers. The protein forms a...Read full article

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    Published: August 2006
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    resonance
    Resonance can be defined in various contexts, including physics, chemistry, and music. Here are definitions for resonance in a few different fields: Physics: In physics, resonance refers to the phenomenon where an oscillating system or object exhibits an increase in amplitude at its natural frequency when subjected to external forces or vibrations. This can occur when the frequency of an external force matches the natural frequency of the system, leading to a resonance effect. Chemistry: In...
    BiophotonicsDNAfilamentfluorescenceFRETHaHHMIHoward Hughes Medical InstituteIllinoisJooMcKinneyNews & FeaturesproteinRECArecombinationresonance

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