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Dark states of monomeric red fluorescent proteins studied

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Care must be taken when analyzing fluorescence correlation spectroscopy of these proteins.

Hank Hogan

You don’t have to be afraid of the dark, but you should be aware of it, according to researchers from Katholieke Universiteit Leuven in Belgium. In a study, they found that the monomeric red fluorescent proteins mRFP1, mCherry and mStrawberry all exhibit a flickering behavior because they enter and exit light-enhanced dark states. Some of the flickering is on a timescale close to that of diffusion, which can make it hard to make sense of experimental measurements. Graduate student Jelle Hendrix, a research team member, has had some firsthand experience with this problem, having run into...Read full article

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    Published: April 2008
    BiophotonicsenergyfluorescentMicroscopymicrosecondResearch & Technologyspectroscopy

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