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Revealing How Cells Die

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BUFFALO, N.Y., Aug. 27, 2010 — Apoptosis, or programmed cell death, is essential to normal development, healthy immune system function and cancer prevention. The process dramatically transforms cellular structures, but the limitations of conventional microscopy methods have kept much about this structural reorganization a mystery. Now, scientists at the University at Buffalo have developed a biophotonic imaging approach capable of real-time monitoring of the transformations that cellular macromolecules undergo during apoptosis. The work, which is featured in a recent issue of PNAS, could help realize the potential of...Read full article

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    Published: August 2010
    Glossary
    coherent anti-stokes raman scattering
    A technique whereby two laser beams, one at an excitation wavelength and the second at a wavelength that produces Stokes Raman scattering, interact coherently in a sample, producing a strong scattered beam at the anti-Stokes wavelength.
    two-photon excited fluorescence
    Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living tissue. The advantage of TPEF in comparison to conventional fluorescence microscopy is that it provides natural confocality and allows sectioning of the sample. Because it typically uses near-infrared excitation light, the penetration depth is significantly increased. TPEF is implemented as fast imaging microscopy for noninvasive optical pathology. TPEF has been used in...
    Aliaksandr V. KachynskiAmericasAndrey KuzminApoptosisArtem PlissBiophotonicsBiophysical Journalcancercell cyclecellular structurecoherent anti-Stokes Raman scatteringDNAenergyfluorescence recovery after photobleachingILPBImagingimmune systemInstitute for LasersLight SourceslipidsmacromoleculesMicroscopymolecular medicinemultiplex imagingParas N. PrasadPhotonics and BiophotonicsPNASproteinsResearch & TechnologyRNAtwo-photon excited fluorescenceLEDs

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