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STED Microscopy: A New Chapter in Light Imaging

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Marie Freebody, Contributing Editor, [email protected]

When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using STED microscopy to smash through more boundaries in fundamental science. Looking back over two decades of progress, the man behind the revolutionary light imaging technique says he is very satisfied with how STED (stimulated emission depletion) microscopy has evolved our understanding of biomolecules as well as transformed the way we think about how light behaves. “As a result of this...Read full article

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    Published: September 2013
    Glossary
    fluorescence correlation spectroscopy
    A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in solution or membranes. FCS measurements rely on recording the transition of several thousands of molecules through the focal volume. The combination of short measurement times along with free positioning or scanning of the observation spot makes FCS an excellent tool for investigating diffusion heterogeneity over time and space.
    sted microscopy
    STED microscopy, or stimulated emission depletion microscopy, is a super-resolution imaging technique in fluorescence microscopy that surpasses the diffraction limit, enabling the visualization of structures at the nanoscale level. This technique was developed to overcome the limitations imposed by the diffraction of light, which traditionally hindered the resolution of optical microscopy to a few hundred nanometers. Key features and principles of STED microscopy: Super-resolution: STED...
    stochastic optical reconstruction microscopy
    Stochastic optical reconstruction microscopy (STORM) is a super-resolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional optical microscopy. It falls under the category of single-molecule localization microscopy (SMLM) methods. STORM was first introduced in 2006 and has since become a powerful tool in biological research for visualizing fine details of cellular structures. The key principle behind STORM involves...
    Basic ScienceBiophotonicscell biologyChristian Eggelingcontinuous-wave stimulated emission depletioncorrelation spectroscopyCW-STED microscopyDengue fever virusDENVenergyFeaturesfluorescence correlation spectroscopygreen fluorescent proteinground state depletion imagingGSDIMHCVHepatitis C virusHIVhuman immunodeficiency virusImaginginfluenza virusJochen SieberLeica Microsystems GmbHlive brain imagingMarie Freebodymaterial sciencesMax Planck Institute for Biophysical ChemistryMembrane researchNature MethodsPALMPhoto Activated Localization MicroscopyphotoswitchingRESOLFTsaturated structured-illumination microscopySSIMSTED microscopySTED superresolutionSTED-FCSStefan Helstimulated emission depletion microscopystochastic optical reconstruction microscopySTORMyellow fluorescent protein

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