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Spinning-disk microscope peers into the heart of a cell

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A new microscopy technique with unprecedented focusing ability allows structures deep within cells – even inside viruses and bacteria – to be investigated for the first time. Advances in optical physics, such as superresolution microscopy, have made it possible to use fluorescence to study complex structures smaller than 200 nm; however, only the structures at the bottom of the cell can be imaged clearly with these techniques. The nucleus and other vital information are in the middle of the cell, while bacterial and viral infections are scattered throughout, imposing...Read full article

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    Published: December 2013
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    probe
    Acronym for profile resolution obtained by excitation. In its simplest form, probe involves the overlap of two counter-propagating laser pulses of appropriate wavelength, such that one pulse selectively populates a given excited state of the species of interest while the other measures the increase in absorption due to the increase in the degree of excitation.
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