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STED Microscopy Made Easy

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Olaf Schulz and Marcelle Koenig, Picoquant

Stimulated emission depletion microscopy surpasses diffraction-limited microscopy by offering much more information about structures. Combining STED with time-tagged data acquisition methods makes even more information available. With fluorescence microscopy, cellular structures and proteins of interest can be imaged with very high detail; fluorescent proteins even enable detailed investigation of live cells. One drawback of fluorescence microscopy is the limited spatial resolution of optical microscopy. In his seminal paper in 1873, Abbe explained why an optical microscope cannot...Read full article

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    Published: December 2014
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    sted microscopy
    STED microscopy, or stimulated emission depletion microscopy, is a super-resolution imaging technique in fluorescence microscopy that surpasses the diffraction limit, enabling the visualization of structures at the nanoscale level. This technique was developed to overcome the limitations imposed by the diffraction of light, which traditionally hindered the resolution of optical microscopy to a few hundred nanometers. Key features and principles of STED microscopy: Super-resolution: STED...
    spatial resolution
    Spatial resolution refers to the level of detail or granularity in an image or a spatial dataset. It is a measure of the smallest discernible or resolvable features in the spatial domain, typically expressed as the distance between two adjacent pixels or data points. In various contexts, spatial resolution can have slightly different meanings: Imaging and remote sensing: In the context of satellite imagery, aerial photography, or other imaging technologies, spatial resolution refers to the...
    near-field scanning
    A measurement technique used to determine the spatial distribution profile of an electrical or optical quantity of interest which is provided by multiple scans within the near-field region of a component or device under test. In optics, this technique is used for measuring the index profile of an optical fiber by illuminating the entrance face with an extended source and measuring the point-by-point radiance of the exit face.
    FeaturesFiltersmirrorssuperresolutionOpticsImagingMicroscopyBiophotonicsEuropestimulated emission depletion microscopySTED microscopyfluorescent proteinsspatial resolutionoptical microscopynear-field scanningphotoactivated localization microscopystochastic optical reconstructionDr. Stefan W. HellDr. Eric BetzigDr. W.E. Moernergated STEDOlaf SchulzMarcelle Koenig

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