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Fluorescent Label Aids Whole-Brain Imaging In Vivo

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A new permanent fluorescent label allows researchers to study complex neural activity in wide swaths of brain tissue in moving animals. Developed at the Howard Hughes Medical Institute, the label frees scientists from the need to use a microscope to observe neuronal activity. Called CaMPARI, for calcium-modulated photoactivatable ratiometric integrator, the label has been tested in living mice, fruit flies and zebrafish. “The most enabling thing about this technology may be that you don’t have to have your organism under a microscope during your experiment,” said...Read full article

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    Published: February 2015
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    Research & TechnologyAmericasVirginiaHoward Hughes Medical CenterJanelia Research CampusHHMIJaneliaLoren LoogerBen FosqueEric SchreiterImagingBiophotonicsfluorescenceBioScanTech Pulse

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