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Live-Cell Imaging Thrives With Microenvironment Control Systems

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As microscopy techniques become more complex and require researchers to keep specimens under objectives for longer periods, microenvironmental control systems play an important role in pushing the limits of live-cell imaging.

DANIEL FOCHT, BIOPTECHS INC.

Choosing a microenvironmental control system for a microscope is not a trivial matter. The results of a live-cell imaging experiment should not be compromised by any part of the data acquisition process. Typically, live-cell experiments can be classified into two categories: developmental studies to establish natural behavior and induced change to study the effects of a controlled factor. Either way, advanced microenvironmental control systems are increasingly playing an important role in breakthroughs in cell biology, in vitro fertilization, tissue engineering, oncology research, neurology...Read full article

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    Published: August 2016
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    microenvironmental control system
    Equipment that supports microscopic live-cell imaging by managing the gas, media perfusion, temperature and other factors to which a sample is exposed. While reducing the negative impacts these factors can have on optical quality, the system also fosters the environment in which a specimen normally lives and allows for the observation of changes to it through the introduction of variables.
    sted microscopy
    STED microscopy, or stimulated emission depletion microscopy, is a super-resolution imaging technique in fluorescence microscopy that surpasses the diffraction limit, enabling the visualization of structures at the nanoscale level. This technique was developed to overcome the limitations imposed by the diffraction of light, which traditionally hindered the resolution of optical microscopy to a few hundred nanometers. Key features and principles of STED microscopy: Super-resolution: STED...
    MicroscopysuperresolutionBiophotonicsAmericasmicroenvironmental control systemconfocal microscopymultiphoton microscopystimulated emission depletion microscopySTED microscopyserial plane deconvolution microscopysuperresolution microscopyflow cellBioptechsDaniel FochtFeatures

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