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Knife-Edge Scanning Microscope

Knife-Edge Scanning Microscope (KESM): An imaging device originally created to image whole mouse brain volumes at microscopic resolution. The main component of the instrument is an automated microtome and microscope capable of producing large numbers of images in a short period of time. Prestained tissue blocks are sectioned by a diamond knife that is back-illuminated with a light source. As sections move over the knife illumination, they are scanned by the integrated microscope, producing volumetric image datasets with high co-registration fidelity.
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