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HILIC Columns

Photonics.com
Jun 2010

 
MILFORD, Mass., June 30, 2010 — Thermo Fisher Scientific Inc.’s Chromatography Consumables & Specialty Products Division is launching a line of hydrophilic interaction liquid chromatography (HILIC) columns. The Hypersil Gold HILIC columns are a technique for separating highly polar compounds that are difficult to analyze using standard reversed-phase chromatography.

With the ability to retain highly polar and hydrophilic compounds, the columns were developed to aid the analysis of compounds that are difficult to retain using conventional C18 columns. Available in 1.9-, 3- and 5-µm particle sizes, they have applications in drug discovery, food testing and environmental analysis.

HILIC works by incorporating water into the highly organic mobile phase to form an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. The highly organic mobile phase contains low salt levels, making the columns suitable for use with electrospray mass spectroscopy.

For more information, visit: www.thermoscientific.com


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