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SymPhoTime Software 5.2.4
Mar 2011
PicoQuant GmbHRequest Info
BERLIN, March 2, 2011 — PicoQuant GmbH has released SymPhoTime software version 5.2.4. The fluorescence lifetime imaging and correlation software now supports the eight-channel configuration of its HydraHarp 400 time-correlated single-photon-counting system (TCSPC) and, therefore, data acquisition and analysis for up to eight detection channels. Along with the release, the company has started a dedicated user discussion forum on the Internet.

Another enhancement is the extension of the measurement preview functions. It is now possible to display TCSPC histograms as well as intensity time traces for each channel separately during the measurements. This is advantageous for Förster resonance energy transfer measurements because it enables direct monitoring of changes in the fluorescence intensity of the donor and acceptor during data acquisition.

The online fluorescence lifetime imaging microscopy features of the software also have been improved by adding a scalable intensity and lifetime color code, increasing image contrast and enabling quick assessment of image quality during measurement.

The “SymPhoTime forum” is targeted at discussions and support about the software scripting language STUPSLANG; however, support on other software functions or discussions about the scientific background also are welcome. The forum can be reached at

The SymPhoTime software package is designed to analyze time-tagged time-resolved measurement data acquired with the proprietary TimeHarp 200, PicoHarp 300 or HydraHarp 400 TCSPC units. It is also the operation and analysis software of the confocal microscope MicroTime 200 as well as the upgrade kit for laser scanning microscopes. SymPhoTime is designed for integration of a broad range of algorithms and methods for the analysis of fluorescence dynamics that users may require.


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BiophotonicsEuropeFLIMfluorescence lifetime imagingFörster resonance energy transferFREThistogramsHydraHarp 400imagingintensity time tracesMicroscopyMicroTime 200online fluorescence lifetime imaging microscopyPicoHarp 300PicoQuantProductsSoftwareSTUPSLANGSymPhoTime 5.2.4TCSPCtime-correlated single-photon-counting systemtime-tagged time-resolvedTimeHarp 200

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