Close

Search

Search Menu
Photonics Media Photonics Buyers' Guide Photonics EDU Photonics Spectra BioPhotonics EuroPhotonics Industrial Photonics Photonics Showcase Photonics ProdSpec Photonics Handbook
More News
share
Email Facebook Twitter Google+ LinkedIn
PROMOTED
CONTENT
Promoted content

Light Sheet Microscopy (oSPIM)

Applied Scientific Instrumentation Inc.Request Info
 
Applied Scientific Instrumentationnnnn's Light Sheet Microscopy (oSPIM)ASI’s Oblique Single Illumination Microscope (oSPIM) is an excellent platform for high resolution light sheet microscopy for samples mounted in standard coverslip-bottom culture dishes. The oSPIM is a single-view light sheet system where the illumination light sheet is generated at an oblique angle using an oil immersion objective below the sample dish. Fluorescent emission is observed using a high NA water dipping objective from the top side where this objective is tilted 60 degrees, perpendicular to the illumination sheet. The arrangement with high NA objectives both above and below the sample dish allow for high-resolution imaging in a convenient geometry for cell culture work.

The oSPIM is two microscopes in one. The lower microscope can be used for fluorescent imaging using conventional modalities, such as wide field fluorescence, confocal, or TIRF. It also is utilized as the light sheet excitation objective. The tilted top microscope is dedicated to Selective Plane Illumination Microscopy (SPIM) or light sheet images.

oSPIM Features:

   •   Low photobleaching: >10x reduction vs. confocal/spinning disk
   •  Water dipping emission objective yields 280 nm transverse resolution
   •  Rapid 3D imaging at oblique angle to coverslip, up to 200 planes per second
   •  Conventional sample mounting in cell culture dishes
   •   Fully functional “conventional” fluorescent microscope in addition to the light sheet modality
   •  Modular and flexible


REQUEST INFO ABOUT THIS PRODUCT

* Message:
(requirements, questions for supplier)
Your contact information
* First Name:
* Last Name:
* Email Address:
* Company:
Address:
Address 2:
City:
State/Province:
Postal Code:
* Country:
Phone #:
Fax #:

Register or login to auto-populate this form:
Login Register
* Required
ProductsBiophotonicsApplied Scientific InstrumentationMicroscopyHigh Resolution Light SheetRapid 3D Imagingcell cultureSelective Plane Illumination

Terms & Conditions Privacy Policy About Us Contact Us
back to top

Facebook Twitter Instagram LinkedIn YouTube RSS
©2017 Photonics Media
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
X
Are you interested in this product?
If you'd like Applied Scientific Instrumentation Inc. to reach out to you with more information about this product, please supply your email and they will contact you.

Email Address:
Stop showing me this for the remainder of my visit