Request InfoOlympus Europa SE & Co. KGHAMBURG, Germany, July 26, 2012 — Olympus Europa Holding GmbH has launched the NA 1.7 APON100×HOTIRF objective for imaging intracellular molecules. With a numerical aperture of 1.7, the objective achieves high Z-resolution for total internal reflection fluorescence (TIRF) microscopy, and offers thin penetration depth and a high signal-to-noise ratio. The lens is suitable for use in single-molecule dynamic studies, fluorescence detection and superresolution applications. It combines a special sapphire glass and a low-autofluorescence (reduced noise) immersion oil that has a high index of refraction. It improves...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Mar/Apr 2024 IssueSuperresolution SIM MicroscopesThe Lattice SIM 3 and Lattice SIM 5 from ZEISS are superresolution microscopes that use structured illumination microscopy (SIM) to overcome the physical resolution limits of light microscopy. 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The event will feature several 15-minute online presentations on the use of light-based...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarytotal internal reflection fluorescenceTotal internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the principle of total internal reflection to selectively illuminate and image...Photonics DictionarySTED microscopySTED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence microscopy that surpasses the diffraction limit, enabling the visualization of...Photonics Dictionarystochastic optical reconstruction microscopyStochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional...Photonics DictionarysuperresolutionSuperresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. 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