Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a very versatile microscopy method where fluorescence lifetime information is combined with spatial localization in the sample, allowing investigating, for example, biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition is typically based on Time-Correlated Single Photon Counting (TCSPC) electronics, pulsed diode lasers, and highly sensitive single photon counting detectors. Up to now TCSPC...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. The integration can overlay both FLIM and Raman images...Promoted Content November 2022 IssueRapid Automated Modular MicroscopeRapid Automated Modular Microscope is mounted on the RAMM frame that supports the microscope assembly and the stage in a manner that ensures close coupling between sample and objective to minimize...Photonics.com 6/12/2023Surface Analysis SoftwareThe Mountains® 10 surface metrology and microscopy analysis software from Digital Surf introduces new features and tools to enhance analysis capabilities. The MountainsImage® product...BioPhotonics Jul/Aug 2022 IssuePhoton-Counting Sensor and CameraGigajot Technology Inc.’s QIS41 camera, built around the GJ04122 sensor, pairs well with standard 4/3-in. microscopy optics, bringing high resolution and low-light performance to scientific and...ARTICLESBioPhotonics May/Jun 2023 IssueStress tests borne of mice and menMen and women respond differently to stimuli, and their hearts respond differently, too. Scientists are continuing to develop an understanding as to why these disparities in physical reactions exist....BioPhotonics Nov/Dec 2022 IssueFlow Cytometry Technique Shows Promise for Early Cancer DiagnosisA digital droplet microfluidic flow cytometry technique could enable earlier recognition of tumor development and progress, by aiding in the detection of multiple microRNAs (miRNAs) in single...BioPhotonics Sep/Oct 2022 IssueA Multiphoton Microscope Enables Portable 3D Biological ImagingFor decades, optical microscopy has provided the mechanism with which to image cells and tissue for the purposes of cancer research, digital pathology, and the study of the brain. Samples are...BioPhotonics Jan/Feb 2023 IssueNobel Prize-Winning Techniques Help Resolve Imaging ChallengeUsing a technique that was recently awarded the 2022 Nobel Prize in chemistry, researchers at Cornell University used expansion microscopy to study lipids, the water-repellent, dynamic components...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookSuperresolution Microscopy: An Imaging RevolutionSuperresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookInfrared Spectral Selection: It Begins with the DetectorSpectral selection is a powerful tool that enhances conventional imaging tremendously. Most imaging systems, including the human eye, are designed to image light over a broad range of the spectrum....Photonics HandbookImage Intensification: The Technology of Night VisionImage intensification, the basis of night vision, is a complex conversion of energy particles that occurs within a vacuum tube. An image-intensifier system works by collecting photons through an...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 2/28/2024Multi-Bandpass Filters for FluorescenceLearn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.Photonics.com 10/4/2022Product Development Strategies for Electro-Optical SystemsThis whitepaper looks at the unique challenges associated with optical systems by exploring two extremes, an ultra-high precision instrument, and a low-cost consumer product, and the different...Photonics.com 8/17/2022Fluorescence, Delayed Fluorescence and Phosphorescence Spectra of a TADF Emitter Measured Using the FLS1000 with a VPL Laser and Gated PMT DetectorMaterials exhibiting thermally activated delayed fluorescence (TADF) have attracted widespread research attention as a new generation of high-efficiency emitters for use in Organic Light Emitting...WEBINARSPhotonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. Emphasis is placed on the integration of FLIM in surgical...Photonics.com 10/26/2022Getting Outstanding Results From Multispectral TCSPC Fluorescence Lifetime ImagingHeitz discusses how scientific results from various cutting-edge studies performed by Becker & Hickl and the company’s collaborators were generated and what to consider when planning...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarysingle-photon avalanche diodeA single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical signals, down to the level of single photons. SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionarylight sheet fluorescence microscopyAlso known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. 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