Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a versatile microscopy method, which combines fluorescence lifetime information with spatial localization, enabling the investigation of biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition based on Time-Correlated Single Photon Counting (TCSPC) is considered a somewhat slow process, due to the time required to collect a sufficient number of photons per pixel for reliable data analysis. ...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. 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The work demonstrates an unconventional way of...Photonics.com 6/27/2022Perovskite Cell, Test Method Set High Bar for Advancing TechnologyResearchers at Princeton University have developed a perovskite solar cell device that can perform above industry standards, maintaining operation for approximately 30 years — compared to the...Photonics.com 2/23/2024SPAD Elevates Spatiotemporal Resolution in Conventional MicroscopesImage scanning microscopy (ISM), a superresolution technique enabled by the advent of fast and compact detector arrays, provides signal-to-noise ratio (SNR), optical sectioning, and spatial...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookSuperresolution Microscopy: An Imaging RevolutionSuperresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookMicroscopy Illumination: Considering Nonlaser Light SourcesAs recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...Photonics HandbookAspheric Lenses: Design ConsiderationsAspheric surfaces are powerful tools that combine the optical corrections of multiple lenses into a single element (Figure 1) and affect performance in ways that spherical optics cannot. For example,...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 10/4/2022Product Development Strategies for Electro-Optical SystemsThis whitepaper looks at the unique challenges associated with optical systems by exploring two extremes, an ultra-high precision instrument, and a low-cost consumer product, and the different...Photonics.com 2/28/2024Multi-Bandpass Filters for FluorescenceLearn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.Photonics.com 4/20/2023Is a DIY Microscope for You?For some advanced microscopy and nanoscopy techniques it becomes necessary to modify existing instrumentation or build an instrument from scratch. This article helps readers determine whether a...WEBINARSPhotonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. 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SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionaryfluorescence lifetime imagingFluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of fluorescence emissions from fluorophores within a sample. Unlike traditional...Photonics Dictionarytime-to-amplitude converterA time-to-amplitude converter (TAC) is an electronic circuit that converts a time interval between two events into an amplitude or voltage signal. 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Its unrivalled...Photonics.com 11/20/2023Andor's Life Science Research SolutionsAt Andor, it matters to us that the products we develop make a real difference to our customers and contribute significantly to their research across Immunology, Microbiology, Neuroscience, Cells &...Photonics.com 11/20/2023New ZL41 Cell & Sona sCMOS - Easy, Flexible, Cost-Effective Super Resolution Microscopy - Andor TechnologyEasy and Cost-Effective Super-Resolution to ~100 nm. Andor has introduced two new highly innovative sCMOS cameras, both delivering easy and cost-effective access to super-resolution capabilities and...PHOTONICS BUYERS' GUIDE CATEGORIESPicosecond LasersStreak CamerasA camera streak is a high-speed cine camera used to record, on a continuously moving film, very brief events such as a flash of light. 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DK Photonics produces a large array of...Photonics Buyers' GuideLaserPoint srlLaserPoint manufactures a wide portfolio of laser power and energy meters able to perform laser measurements with high accuracy. They developed the hard coatings to absorb the laser radiation which...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...