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rapidFLIM: The New and Innovative Method for Ultra fast FLIM Imaging

Author: Sandra Orthaus-Mueller, Ben Kraemer, Rhys Dowler, André Devaux, Astrid Tannert, Tino Roehlicke, Michael Wahl, Hans-Juergen Rahn and Rainer Erdmann
Tuesday, August 08, 2017
rapidFLIM exploits recent advances in Time-Correlated Single Photon Counting (TCSPC) electronics, where ultra-short dead times allows imaging fast, dynamic processes via Fluorescence Lifetime Imaging (FLIM). With this new approach, FLIM image acquisition with more than 10 frames per second is possible, enabling imaging of dynamic processes (protein interactions, chemical reactions), highly mobile species (mobility of cell organelles or particles, cell migration), and studying FRET dynamics.
File: AppNote_rapidFLIM.pdf (1.83 MB)
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BiophotonicsPicoQuant GmbHrapidFLIMFLIMFRETimagingfluorescence lifetimeTCSPCMicroscopytime-resolvedlife sciences
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