Munich, Germany, August 1 -- Photonics GmbH announced that its Microimager beamsplitter module for simultaneous dual emission imaging is ready for delivery.
The Microimager creates two spectrally different images on the CCD chip, allowing the microscope operator to capture multiple images without having to change filters. Typical applications for the beamsplitter are indo calcium imaging and CFP/YFP FRET fluorescence-resonance-energy-transfer -- where two biological molecules are labeled with different dyes -- donor fluorophore and acceptor fluorophore. Donor-fluorophore is excited with narrow band illumination and stimulates the fluorescence of the donor-labeled molecule. The donor transfers its energy without emission to the acceptor labeled molecule as it comes into close proximity of the donor-labeled molecule. As a result of this energy transfer, the donor fluorescence will be quenched and the acceptor fluorescence is stimulated to emit a fluorescence photon.