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DataRay Inc. - ISO 11146-Compliant Laser Beam Profilers

Is Vibration Control Really Necessary for Microscopy?

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James Fisher, Newport Corporation

As a supplier of vibration control equipment, we at Newport are frequently asked if vibration control is really necessary for microscopy applications, and you might expect the answer to be “of course.” However, the correct response is slightly more complex because the need for vibration control lies between the environmental conditions in the laboratory and the requirements of the experiment. The lab environment Identifying and quantifying the potential noise sources in your lab are the first steps toward understanding what vibration control solutions may be required to...Read full article

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    Published: July 2010
    Glossary
    beam
    1. A bundle of light rays that may be parallel, converging or diverging. 2. A concentrated, unidirectional stream of particles. 3. A concentrated, unidirectional flow of electromagnetic waves.
    coherent anti-stokes raman scattering
    A technique whereby two laser beams, one at an excitation wavelength and the second at a wavelength that produces Stokes Raman scattering, interact coherently in a sample, producing a strong scattered beam at the anti-Stokes wavelength.
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    footprint
    1. The sector of the Earth's surface registered upon a remote sensing device in a satellite. 2. The amount of space occupied by a component on the surface upon which it is mounted. 3. The space on an optical component occupied by a light beam.
    isolator
    In the context of electronics and optics, an isolator refers to a device that allows the transmission of signals in one direction while attenuating or blocking signals in the opposite direction. The primary purpose of an isolator is to protect components or systems from undesired reflections and signals that may cause interference or damage. Two common types of isolators and their applications are: Electrical isolator: Function: An electrical isolator, also known as an electrical or power...
    microscope
    An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
    noise
    The unwanted and unpredictable fluctuations that distort a received signal and hence tend to obscure the desired message. Noise disturbances, which may be generated in the devices of a communications system or which may enter the system from the outside, limit the range of the system and place requirements on the signal power necessary to ensure good reception.
    optical
    Pertaining to optics and the phenomena of light.
    positioning
    Positioning generally refers to the determination or identification of the location or placement of an object, person, or entity in a specific space or relative to a reference point. The term is used in various contexts, and the methods for positioning can vary depending on the application. Key aspects of positioning include: Spatial coordinates: Positioning often involves expressing the location of an object in terms of spatial coordinates. These coordinates may include dimensions such as...
    spectrometer
    A kind of spectrograph in which some form of detector, other than a photographic film, is used to measure the distribution of radiation in a particular wavelength region.
    Air Compressoranti-Stokesautomotivebeambiological imagingBiophotonicsbuilding resonanceCARSCARS microscopychemical analysiscoherent anti-Stokes Raman scatteringelevatorFeaturesfluorescencefluorescence microscopyFootprintHVACImagingimaging systemsin vivo imagingIsolatorJames FisherlaboratoryMaker and TehuneMicronmicroscopeMicroscopymyelin fibersNewDamp elastomer isolatorNewportNewport Corp.Newport RS2000noisenoise sourcesnonlinearopticalOpticsOTS platformplatform vibrationpneumatic isolatorpositioningpositioning stagepumprotating deviceS-2000sample labelingSL-1200SmartTable OTSspatial misalignmentspatial positionSpectrometerspectroscopyStokes pulsestarget point stabilityTechnology Applications CenterTest & Measurementtwo-photon excited fluorescence microscopyVC-Bvibrationvibration controlvibration criteria curvevibration isolationvibration standard criteriaVison IsoStationXieLasers

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