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Photonics Handbook

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Superresolution Microscopy: An Imaging RevolutionSuperresolution Microscopy: An Imaging Revolution
MARIE FREEBODY, CONTRIBUTING EDITOR, [email protected]
Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...
Fluorescence Lifetime Imaging: Choosing the Best ApproachFluorescence Lifetime Imaging: Choosing the Best Approach
GERHARD HOLST, Excelitas PCO GmbH
The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...
Microscopy Illumination: Considering Nonlaser Light SourcesMicroscopy Illumination: Considering Nonlaser Light Sources
BARBARA FOSTER, THE MICROSCOPY & IMAGING PLACE INC.
As recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...
Lasers for Microscopy: Major TrendsLasers for Microscopy: Major Trends
MARCO ARRIGONI, NIGEL GALLAHER, DARRYL MCCOY, VOLKER PFEUFER, MATTHIAS SCHULZE, AND DANIEL CALLEN, COHERENT INC.
Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...
Nd:YAG Lasers: Standing the Test of TimeNd:YAG Lasers: Standing the Test of Time
Quantel USA
The ubiquitous Nd:YAG laser has played many roles over the years. For the military, it has provided rangefinding and target designation capabilities. When used with nonlinear optics or as a pump...
Tunable Lasers: Generating Wavelengths from the UV Through the IRTunable Lasers: Generating Wavelengths from the UV Through the IR
Ian Read, MKS/Spectra-Physics
Applications facilitated by tunable lasers fall into two categories: situations in which one or more discrete wavelengths are not available from any single- or multiline fixed-wavelength laser, or...
Spectroscopy: The Tools of the TradeSpectroscopy: The Tools of the Trade
Dr. John R. Gilchrist, Clyde HSI
All optical spectrometry techniques rely on the measurement of radiant power. The configuration of the instrument varies based on the measurement technique: absorption, emission, luminescence, or...
Particle Image Velocimetry: Basics, Developments and TechniquesParticle Image Velocimetry: Basics, Developments and Techniques
M. Kelnberger, InnoLas GmbH; G. Schwitzgebel, Universität Mainz
Particle image velocimetry (PIV) is an experimental tool in fluid mechanics and aerodynamics. The basic principle involves photographic recording of the motion of microscopic particles that follow...
Photonics Handbook

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